| Influence of global sperm DNA methylation on IVF results. | |
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MedLine Citation:
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PMID: 15640258 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In cases of male infertility, routine analysis for sperm characteristics is a poor predictive factor for the segmentation rate and embryo development in assisted reproductive technologies. It is assumed that epigenetic factors could have an influence on the embryo's quality. The aim of this work was to determine the relationship between sperm DNA methylation level and fertilization and pregnancy rates according to the assisted reproduction technique performed. METHODS: A prospective study was undertaken. Ejaculates were obtained from men (n = 63) undergoing an assisted reproduction procedure. 5-Methylcytosine was immunostained with a polyclonal antibody and revealed by fluorescein isothiocyanate. The DNA methylation level was then quantified by flow cytometry. RESULTS: Sixty-three conventional IVF cycles were performed, 760 oocytes were retrieved, an average of 8.1 +/- 4.8 embryos was obtained, and 2.4 embryos were transferred. Neither the fertilization rate nor the rate of good quality embryos was correlated with the DNA methylation level (r = -0.1 and r = -0.08 respectively; not significant). When sperm DNA methylation was >555 arbitrary units, the pregnancy rate was 33.3% compared with 8.3% in the lower (<555) group (P<0.05). CONCLUSION: DNA methylation level in human sperm could represent a new approach to study the ability of sperm to lead to pregnancy in an assisted reproduction procedure, especially when sperm samples with normal characteristics are used. |
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Authors:
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M Benchaib; V Braun; D Ressnikof; J Lornage; P Durand; A Niveleau; J F Guérin |
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Publication Detail:
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Type: Journal Article Date: 2005-01-07 |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 20 ISSN: 0268-1161 ISO Abbreviation: Hum. Reprod. Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2005-02-18 Completed Date: 2005-07-21 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 768-73 Citation Subset: IM |
Affiliation:
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Département de Médecine de la Reproduction, Hôpital Edouard Herriot, place d'Arsonval, 69373 Lyon cedex 08, France. mehdi.benchaib@sante.univ-lyon1.fr |
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| MeSH Terms | |
Descriptor/Qualifier:
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5-Methylcytosine
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metabolism DNA Methylation* Embryo Transfer Embryo, Mammalian / physiology Female Fertilization Fertilization in Vitro* Flow Cytometry Fluorescein-5-isothiocyanate Fluorescent Dyes Humans Immunologic Techniques Male Pregnancy Pregnancy Rate Prospective Studies Spermatozoa / metabolism, physiology* Staining and Labeling Treatment Outcome |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 3326-32-7/Fluorescein-5-isothiocyanate; 554-01-8/5-Methylcytosine |
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