Document Detail

Influence of global sperm DNA methylation on IVF results.
MedLine Citation:
PMID:  15640258     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: In cases of male infertility, routine analysis for sperm characteristics is a poor predictive factor for the segmentation rate and embryo development in assisted reproductive technologies. It is assumed that epigenetic factors could have an influence on the embryo's quality. The aim of this work was to determine the relationship between sperm DNA methylation level and fertilization and pregnancy rates according to the assisted reproduction technique performed. METHODS: A prospective study was undertaken. Ejaculates were obtained from men (n = 63) undergoing an assisted reproduction procedure. 5-Methylcytosine was immunostained with a polyclonal antibody and revealed by fluorescein isothiocyanate. The DNA methylation level was then quantified by flow cytometry. RESULTS: Sixty-three conventional IVF cycles were performed, 760 oocytes were retrieved, an average of 8.1 +/- 4.8 embryos was obtained, and 2.4 embryos were transferred. Neither the fertilization rate nor the rate of good quality embryos was correlated with the DNA methylation level (r = -0.1 and r = -0.08 respectively; not significant). When sperm DNA methylation was >555 arbitrary units, the pregnancy rate was 33.3% compared with 8.3% in the lower (<555) group (P<0.05). CONCLUSION: DNA methylation level in human sperm could represent a new approach to study the ability of sperm to lead to pregnancy in an assisted reproduction procedure, especially when sperm samples with normal characteristics are used.
M Benchaib; V Braun; D Ressnikof; J Lornage; P Durand; A Niveleau; J F Guérin
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Publication Detail:
Type:  Journal Article     Date:  2005-01-07
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  20     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-18     Completed Date:  2005-07-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  768-73     Citation Subset:  IM    
Département de Médecine de la Reproduction, Hôpital Edouard Herriot, place d'Arsonval, 69373 Lyon cedex 08, France.
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MeSH Terms
5-Methylcytosine / metabolism
DNA Methylation*
Embryo Transfer
Embryo, Mammalian / physiology
Fertilization in Vitro*
Flow Cytometry
Fluorescent Dyes
Immunologic Techniques
Pregnancy Rate
Prospective Studies
Spermatozoa / metabolism,  physiology*
Staining and Labeling
Treatment Outcome
Reg. No./Substance:
0/Fluorescent Dyes; 3326-32-7/Fluorescein-5-isothiocyanate; 554-01-8/5-Methylcytosine

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