| Influence of gadolinium-induced kupffer cell blockade on portal venous tolerance in rat skin allograft transplantation. | |
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MedLine Citation:
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PMID: 15818041 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Intraportal injection of donor antigens delays rejection of allografts (portal venous tolerance). The study aimed to investigate the possible influence of prior gadolinium chloride (Gd)-induced Kupffer cell blockade on tolerance to non-vascularized skin allografts induced by means of donor-specific intraportal blood transfusion. MATERIALS AND METHODS: Wistar rats (n = 10) were used as donors and Sprague-Dawley rats (n = 70) as recipients of a non-vascularized skin graft. Recipients were divided into groups according to the manipulations prior to transplantation, as follows: (1) no manipulation; (2) donor-specific intrajugular blood transfusion; (3) donor-specific intraportal blood transfusion; (4) Gd administration and donor-specific intrajugular blood transfusion; (5) Gd administration and donor-specific intraportal blood transfusion; (6) Gd administration, and (7) intraportal saline infusion. In a first set of experiments, these manipulations were performed once. In a second set of experiments, the same manipulations were performed twice. Skin allograft was performed 7 days after the last manipulation in all groups. RESULTS: Group 3 showed the highest skin graft survival, particularly after repeated blood transfusion. Graft survival in this group was significantly higher than in any other group. Conversely, group 5 showed the lowest graft survival, particularly after repeated blood transfusion. Graft survival in this group was significantly lower than that of groups 1, 2, 3 and 7. CONCLUSIONS: In this model of skin allograft transplantation, Gd administration abrogates and can even reverse the tolerogenic effect of repeated donor-specific intraportal blood transfusion. |
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Authors:
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J A Diaz-Peromingo; A Gonzalez-Quintela |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European surgical research. Europäische chirurgische Forschung. Recherches chirurgicales européennes Volume: 37 ISSN: 0014-312X ISO Abbreviation: Eur Surg Res Publication Date: 2005 Jan-Feb |
Date Detail:
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Created Date: 2005-04-08 Completed Date: 2005-07-12 Revised Date: 2006-11-30 |
Medline Journal Info:
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Nlm Unique ID: 0174752 Medline TA: Eur Surg Res Country: Switzerland |
Other Details:
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Languages: eng Pagination: 45-9 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Santiago de Compostela, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Transfusion* Gadolinium / pharmacology* Graft Survival Kupffer Cells / drug effects* Male Portal Vein* Rats Rats, Sprague-Dawley Rats, Wistar Skin Transplantation* Transplantation Tolerance* / drug effects Transplantation, Homologous |
| Chemical | |
Reg. No./Substance:
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10138-52-0/gadolinium chloride; 7440-54-2/Gadolinium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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