| Influence of food deprivation on the transport of 3-O-methyl-alpha-D-glucose across the isolated ruminal epithelium of sheep. | |
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MedLine Citation:
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PMID: 12413097 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent studies provided evidence that the ruminal epithelium is able to absorb D-glucose even at physiologically low intraruminal concentrations. To elucidate whether ruminal D-glucose transport shows adaptive responses during food deprivation, transport of 3-0-methyl-alpha-D-glucose (3-OMG), a hardly metabolizable D-glucose analogue, was measured in isolated ruminal epithelia obtained from hay-fed or food-deprived adult sheep. In both groups, a significant net absorption of 3-OMG to the serosal side (in vivo: blood side oriented) could be detected at 3-OMG concentrations between 0.25 mM and 5 mM. Net absorption of 3-OMG was abolished by mucosal (in vivo: lumen side oriented) addition of phlorizin, an inhibitor of the sodium glucose-linked transporter 1 (SGLT-1). Net absorption of 3-OMG followed Michaelis-Menten kinetics, but apparent affinity and maximal transport capacity were lower in epithelia obtained from food-deprived sheep. In contrast to the decrease of the (secondary) active 3-OMG transport, serosal-to-mucosal permeation of 3-OMG increased after food deprivation, suggesting an elevated passive 3-OMG transfer. It is concluded that the altered transport characteristics are either part of a global energy-sparing process during food deprivation (i.e., a lowered activity of the Na+/K+-ATPase) or result from specific down-regulation of SGLT-1. |
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Authors:
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G Gäbel; J R Aschenbach |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Journal of animal science Volume: 80 ISSN: 0021-8812 ISO Abbreviation: J. Anim. Sci. Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-11-04 Completed Date: 2003-03-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8003002 Medline TA: J Anim Sci Country: United States |
Other Details:
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Languages: eng Pagination: 2740-6 Citation Subset: IM |
Affiliation:
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Veterinär-Physiologisches Institut, Universität Leipzig, Germany. gaebel@rz.uni-leipzig.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3-O-Methylglucose
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pharmacokinetics* Animals Biological Transport / physiology Biological Transport, Active / physiology Female Food Deprivation / physiology* Gastric Mucosa / metabolism Intestinal Absorption Monosaccharide Transport Proteins / antagonists & inhibitors, physiology* Phlorhizin / pharmacology Rumen / metabolism* Sheep / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Monosaccharide Transport Proteins; 146-72-5/3-O-Methylglucose; 60-81-1/Phlorhizin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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