Document Detail

Influence of food deprivation on the transport of 3-O-methyl-alpha-D-glucose across the isolated ruminal epithelium of sheep.
MedLine Citation:
PMID:  12413097     Owner:  NLM     Status:  MEDLINE    
Recent studies provided evidence that the ruminal epithelium is able to absorb D-glucose even at physiologically low intraruminal concentrations. To elucidate whether ruminal D-glucose transport shows adaptive responses during food deprivation, transport of 3-0-methyl-alpha-D-glucose (3-OMG), a hardly metabolizable D-glucose analogue, was measured in isolated ruminal epithelia obtained from hay-fed or food-deprived adult sheep. In both groups, a significant net absorption of 3-OMG to the serosal side (in vivo: blood side oriented) could be detected at 3-OMG concentrations between 0.25 mM and 5 mM. Net absorption of 3-OMG was abolished by mucosal (in vivo: lumen side oriented) addition of phlorizin, an inhibitor of the sodium glucose-linked transporter 1 (SGLT-1). Net absorption of 3-OMG followed Michaelis-Menten kinetics, but apparent affinity and maximal transport capacity were lower in epithelia obtained from food-deprived sheep. In contrast to the decrease of the (secondary) active 3-OMG transport, serosal-to-mucosal permeation of 3-OMG increased after food deprivation, suggesting an elevated passive 3-OMG transfer. It is concluded that the altered transport characteristics are either part of a global energy-sparing process during food deprivation (i.e., a lowered activity of the Na+/K+-ATPase) or result from specific down-regulation of SGLT-1.
G Gäbel; J R Aschenbach
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of animal science     Volume:  80     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-11-04     Completed Date:  2003-03-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2740-6     Citation Subset:  IM    
Veterinär-Physiologisches Institut, Universität Leipzig, Germany.
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MeSH Terms
3-O-Methylglucose / pharmacokinetics*
Biological Transport / physiology
Biological Transport, Active / physiology
Food Deprivation / physiology*
Gastric Mucosa / metabolism
Intestinal Absorption
Monosaccharide Transport Proteins / antagonists & inhibitors,  physiology*
Phlorhizin / pharmacology
Rumen / metabolism*
Sheep / metabolism*
Reg. No./Substance:
0/Monosaccharide Transport Proteins; 146-72-5/3-O-Methylglucose; 60-81-1/Phlorhizin

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