| Influence of fixation and staining techniques on the ultrastructure of the insulin secretory granule. | |
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MedLine Citation:
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PMID: 6205227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ultrastructure of beta-cell granules was examined after fixation of isolated mouse islets in fixatives of various compositions. Islets fixed in glutaraldehyde with or without post-fixing in osmium tetroxide, or in osmium tetroxide alone, exhibited the classical picture of electron-dense core surrounded by a lucid halo. After fixation in potassium permanganate, with or without post-fixing in osmium, the secretory vesicle was often completely filled with a fairly homogeneous material; the vesicle membrane was distinct in outline. When glutaraldehyde-fixed beta-cells were stained according to Rambourg to visualize reducing groups, possibly carbohydrates, two distinct classes of secretory granules were observed, one darkly stained and one faintly, without any noticeable precipitation of silver; the perigranular halo remained unstained in both types of granules. The perigranular halo of aldehyde and osmium fixed beta-granules also remained unstained in cells exposed to the osmiophilic amine, 5,6-dihydroxytryptamine. |
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Authors:
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R Norlund; L Norlund; G D Bloom; I B Täljedal |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Medical biology Volume: 62 ISSN: 0302-2137 ISO Abbreviation: Med. Biol. Publication Date: 1984 |
Date Detail:
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Created Date: 1984-08-27 Completed Date: 1984-08-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417300 Medline TA: Med Biol Country: FINLAND |
Other Details:
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Languages: eng Pagination: 27-33 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cytoplasmic Granules / secretion, ultrastructure* Fixatives Histological Techniques* Insulin / secretion* Islets of Langerhans / secretion, ultrastructure* Mice Microscopy, Electron Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Fixatives; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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