Document Detail

Influence of exogenous interleukin-2 concentration on the isolation of human immunodeficiency virus.
MedLine Citation:
PMID:  7783684     Owner:  NLM     Status:  MEDLINE    
A significant increase (P = 0.015) in the HIV isolation rate from plasma samples was achieved by use of 10 U/ml exogenous interleukin-2 compared to 20 U/ml. The sensitivity rose from 0% to 29% in patients negative for p24 core antigen (P = 0.031) and from 71% to 86% in patients positive for p24 core antigen in plasma (P > 0.05). Titration of infectious HIV revealed that 10 U/ml interleukin-2 is the optimal concentration to isolate low numbers of infectious particles of HIV.
B Zöllner; H H Feucht; A Hamann; R Laufs
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbiology and immunology     Volume:  39     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  1995  
Date Detail:
Created Date:  1995-07-20     Completed Date:  1995-07-20     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  117-21     Citation Subset:  IM; X    
Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Eppendorf, Hamburg, Germany.
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MeSH Terms
Acquired Immunodeficiency Syndrome / blood,  microbiology
Cells, Cultured
DNA, Viral / analysis
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
HIV Core Protein p24 / analysis
HIV Infections / blood*,  microbiology
HIV-1 / genetics,  growth & development,  isolation & purification*
Interleukin-2 / pharmacology*
Leukocytes, Mononuclear / drug effects,  microbiology*
Microbiological Techniques
Polymerase Chain Reaction
Sensitivity and Specificity
Viremia / microbiology
Reg. No./Substance:
0/DNA, Viral; 0/HIV Core Protein p24; 0/Interleukin-2

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