Document Detail

Influence of estrogens on bone resorption in organ culture.
MedLine Citation:
PMID:  1000354     Owner:  NLM     Status:  MEDLINE    
Estradiol benzoate, ethinyl estradiol, and human calcitonin were compared for their ability to inhibit the spontaneous or parathyroid extract-induced bone resorption in organ cultures of 19-day fetal rat fibulae. The criteria used for the assessment of bone resorption were: the release of 45Ca from paired prelabeled bone rudiments into the culture medium, the dry weight, and the number of osteoclasts per bone. Estradiol benzoate at concentrations of 1.3 X 10(-5) to 2.6 X 10(-4)M had no effect on the release of 45Ca from fetal fibulae or on the dry weight. Histologically, treated bones were well preserved and resembled the controls. Parathyroid extract (PTE) alone caused extensive bone resorption with numerous osteoclasts and enhanced 45Ca release without weight gain. The addition of estradiol benzoate to the culture medium did not prevent the resorptive action of PTE. Ethinyl estradiol alone provoked a dose-related inhibition of 45Ca release at concentrations of 3.4 X 10(-5) to 1.7 X 10(-4)M. However, this inhibition was accompanied by a decrease in bone dry weight and by various degrees of cellular damage. The same phenomenon was observed in PTE-treated fibulae. Human calcitonin, on the contrary, inhibited the release of 45Ca by decreasing the number of osteoclasts while the weight of the fibulae increased. The inhibitory action of ethinyl estradiol appears to be caused by a toxic effect on bone cells. It is concluded that estrogens have no direct physiological effect on bone resorption in vitro.
M Liskova
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Calcified tissue research     Volume:  22     ISSN:  0008-0594     ISO Abbreviation:  Calcif Tissue Res     Publication Date:  1976 Dec 
Date Detail:
Created Date:  1977-02-24     Completed Date:  1977-02-24     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0114414     Medline TA:  Calcif Tissue Res     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  207-18     Citation Subset:  IM    
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MeSH Terms
Bone Resorption / drug effects*,  pathology
Bone and Bones / pathology
Calcitonin / pharmacology
Estradiol / pharmacology
Estrogens / pharmacology*
Ethinyl Estradiol / pharmacology
Organ Culture Techniques
Parathyroid Hormone / pharmacology
Reg. No./Substance:
0/Estrogens; 0/Parathyroid Hormone; 50-28-2/Estradiol; 57-63-6/Ethinyl Estradiol; 9007-12-9/Calcitonin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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