Document Detail


Influence of epithelium and isoprenaline incubation on responsiveness of guinea-pig trachea to methacholine.
MedLine Citation:
PMID:  16210880     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There are reports regarding harmful effect of long-term use of beta2-agonist drugs on asthma severity and airway responsiveness. In the present study, the responses of guinea pig trachea with intact and denuded epithelium (groups 1 and 2, n = 10) to methacholine as EC50 were measured in tissues nonincubated or incubated with 10 mumol/l isoprenaline during the resting period. The same protocol was performed in groups 3 and 4 (n = 5 for each group) with an additional 30 min rest time after isoprenaline incubation. The response of trachea with denuded epithelium (groups 2 and 4) to methacholine was significantly higher than that with intact epithelium both in incubated and nonincubated conditions (groups 1 and 3, p < 0.05 to p < 0.001). Incubation with isoprenaline caused a significant reduction in the tracheal response to methacholine in both the denuded groups (p < 0.005 and p < 0.001) and intact epithelium groups (p < 0.005 for both cases). The reduction in tracheal responsiveness to methacholine due to incubation in epithelium denuded trachea (groups 2 and 4) was nonsignificantly greater than that of intact epithelium tissues. There was no difference between groups 3 and 4 with those of groups 1 and 2 in both incubated and non incubated conditions. The maximum contractility response to methacholine was not different between tracheal chains with denuded and intact epithelium and did not change due to incubation with isoprenaline. The results of this study indicate reduction of tracheal response to methacholine due to incubation of tissues with isoprenaline, which was relatively more pronounced in epithelium denuded trachea.
Authors:
M H Boskabady; M R Aslani; A Tabatabaei
Publication Detail:
Type:  In Vitro; Journal Article     Date:  2005-10-04
Journal Detail:
Title:  Pharmacology     Volume:  76     ISSN:  0031-7012     ISO Abbreviation:  Pharmacology     Publication Date:  2006  
Date Detail:
Created Date:  2005-12-06     Completed Date:  2006-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0152016     Medline TA:  Pharmacology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Copyright Information:
Copyright 2006 S. Karger AG, Basel
Affiliation:
Department of Physiology, Ghaem Medical Centre, Mashhad University of Medical Sciences, Mashhad, Iran. mhboskabady@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adrenergic beta-Agonists / pharmacology*
Animals
Asthma / physiopathology
Bronchial Hyperreactivity / chemically induced
Bronchoconstrictor Agents / pharmacology
Bronchodilator Agents / pharmacology*
Epithelium / drug effects,  physiology,  physiopathology
Guinea Pigs
Isoproterenol / pharmacology*
Methacholine Chloride / pharmacology
Muscle Contraction / drug effects
Muscle, Smooth / drug effects,  physiology,  physiopathology
Trachea / drug effects*,  physiology,  physiopathology
Chemical
Reg. No./Substance:
0/Adrenergic beta-Agonists; 0/Bronchoconstrictor Agents; 0/Bronchodilator Agents; 62-51-1/Methacholine Chloride; 7683-59-2/Isoproterenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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