Document Detail


Influence of environmental conditions on the production of phenazine-1-carboxamide by Pseudomonas chlororaphis PCL1391.
MedLine Citation:
PMID:  15141960     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pseudomonas chlororaphis PCL1391 produces the secondary metabolite phenazine-1-carboxamide (PCN), which is an antifungal metabolite required for biocontrol activity of the strain. Identification of conditions involved in PCN production showed that some carbon sources and all amino acids tested promote PCN levels. Decreasing the pH from 7 to 6 or decreasing the growth temperature from 21 to 16 degrees C decreased PCN production dramatically. In contrast, growth at 1% oxygen as well as low magnesium concentrations increased PCN levels. Salt stress, low concentrations of ferric iron, phosphate, sulfate, and ammonium ions reduced PCN levels. Fusaric acid, a secondary metabolite produced by the soilborne Fusarium spp. fungi, also reduced PCN levels. Different nitrogen sources greatly influenced PCN levels. Analysis of autoinducer levels at conditions of high and low PCN production demonstrated that, under all tested conditions, PCN levels correlate with autoinducer levels, indicating that the regulation of PCN production by environmental factors takes place at or before autoinducer production. Moreover, the results show that autoinducer production not only is induced by a high optical density but also can be induced by certain environmental conditions. We discuss our findings in relation to the success of biocontrol in the field.
Authors:
E Tjeerd van Rij; Monique Wesselink; Thomas F C Chin-A-Woeng; Guido V Bloemberg; Ben J J Lugtenberg
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  17     ISSN:  0894-0282     ISO Abbreviation:  Mol. Plant Microbe Interact.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-14     Completed Date:  2004-06-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  United States    
Other Details:
Languages:  eng     Pagination:  557-66     Citation Subset:  IM    
Affiliation:
Leiden University, Institute of Biology, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / pharmacology
Carbon / pharmacology
Ferric Compounds / pharmacology
Fusaric Acid / pharmacology
Hydrogen-Ion Concentration
Ions / pharmacology
Nitrogen / pharmacology
Oxygen / pharmacology
Phenazines / metabolism*
Phosphates / pharmacology
Pseudomonas / drug effects,  growth & development,  metabolism*
Quaternary Ammonium Compounds / pharmacology
Sodium Chloride / pharmacology
Sulfates / pharmacology
Temperature
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Ferric Compounds; 0/Ions; 0/Phenazines; 0/Phosphates; 0/Quaternary Ammonium Compounds; 0/Sulfates; 0/phenazine-1-carboxamide; 536-69-6/Fusaric Acid; 7440-44-0/Carbon; 7647-14-5/Sodium Chloride; 7705-08-0/ferric chloride; 7727-37-9/Nitrogen; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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