| Influence of enteral diets supplemented with key nutrients on lymphocyte subpopulations in Peyer's patches of endotoxin-boostered mice. | |
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MedLine Citation:
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PMID: 10952798 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND AIMS: This study was undertaken to compare the effect of different key nutrients on lymphocyte subsets of Peyer's patches (PP) and spleen in endotoxemic mice. METHODS: Female Balb/c mice were fed over a period of 10 days either with an isocaloric and isonitrogenous control diet (Control), a glutamine enriched diet (Diet I) or a diet containing glutamine, arginine, glycine, and n-3 fatty acids (Diet II). On day 7 the mice were challenged intraperitoneally with 25 microg LPS. The lymphocyte subpopulations (B cells, T cells, CD4+ and CD8+) of PP and spleen were analysed by flow cytometry. Glutathione content of small intestinal mucosa and spleen was determined by HPLC and luminal small intestinal IgA by ELISA. RESULTS: Both experimental diets increased the number of B and T cells in the PP and that of T cells in the spleen (P<0.01). Glutathione content in PP and spleen was higher under administration of key nutrients (P<0.05). Diet II reduced luminal small intestinal IgA content in comparison to the two other groups. CONCLUSION: The addition of arginine, glycine and n-3 fatty acids to a glutamine supplemented diet does not enhance lymphocyte numbers in PP and spleen, but reduces intestinal IgA content. |
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Authors:
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N Manhart; K Vierlinger; R Akomeah; H Bergmeister; A Spittler; E Roth |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical nutrition (Edinburgh, Scotland) Volume: 19 ISSN: 0261-5614 ISO Abbreviation: Clin Nutr Publication Date: 2000 Aug |
Date Detail:
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Created Date: 2000-11-22 Completed Date: 2001-01-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8309603 Medline TA: Clin Nutr Country: SCOTLAND |
Other Details:
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Languages: eng Pagination: 265-9 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Harcourt Publishers Ltd. |
Affiliation:
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Department of Surgical Research, University of Vienna, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid Dietary Supplements* Endotoxemia Enteral Nutrition* Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Glutamine / administration & dosage, blood, immunology Glutathione / administration & dosage, analysis, immunology Immunoglobulin A / metabolism Lymphocytes / drug effects* Mice Mice, Inbred BALB C Peyer's Patches / chemistry, drug effects, immunology* Spleen / chemistry, drug effects, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin A; 56-85-9/Glutamine; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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