| Influence of embedding media in prolactin labelling with immunogold techniques. | |
| | |
MedLine Citation:
|
PMID: 3536804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Immunogold labelling of prolactin in three different embedding media was compared. The polymeric prolactin in secretory granules was labelled in the three media, however, acrylic monomers (Lowicryl K4M and LR White) provided a more intense labelling with higher dilutions of the primary antibody, compared to the labelling in the epoxy resin (araldite). An intense labelling of monomeric prolactin in Golgi complex was detected only in acrylic embedments, and the labelling on the rough endoplasmic reticulum was significant only in LR White embedded tissues. |
| | |
Authors:
|
C A Maldonado; A Aoki |
Related Documents
:
|
3439844 - Light and electron microscopical studies on the appearance of a marginal layer and coll... 7215074 - Microscopic adenoma in nonpolyposis coli: incidence and relation to basal cells and lym... 167574 - The genesis of cell types in the adenohypophysis of the human fetus as observed with im... 2966674 - Secretory morphology of the intermediate lobe of the rat pituitary incubated in vitro. 22991224 - Complex stiffness gradient substrates for studying mechanotactic cell migration. 17325434 - Nifedipine effect on red cell rheological properties in patients with systemic sclerode... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Histochemical journal Volume: 18 ISSN: 0018-2214 ISO Abbreviation: Histochem. J. Publication Date: 1986 Aug |
Date Detail:
|
Created Date: 1987-01-05 Completed Date: 1987-01-05 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0163161 Medline TA: Histochem J Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 429-33 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acrylic Resins* Animals Epoxy Resins* Female Gold Histocytochemistry Immunologic Techniques Phthalic Acids* Phthalic Anhydrides* Pituitary Gland / analysis Prolactin / analysis* Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
|
0/Acrylic Resins; 0/Epoxy Resins; 0/Phthalic Acids; 0/Phthalic Anhydrides; 37226-48-5/araldite; 7440-57-5/Gold; 84137-04-2/Lowicryl 4KM resin; 9002-62-4/Prolactin; 94188-59-7/LR white |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The structure of the basement membrane of human lymph node high endothelial venules: an ultrastructu...
Next Document: Glutathione localization by a novel o-phthalaldehyde histofluorescence method.