| Influence of diet on the proteome of Drosophila melanogaster as assessed by two-dimensional gel electrophoresis and capillary liquid chromatography-mass spectrometry: the hamburger effect revisited. | |
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MedLine Citation:
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PMID: 19137114 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Proteomic biomarker discovery has been called into question. Diamandis hypothesized that seemingly trivial factors, such as eating a hamburger, may cause sufficient proteomic change as to confound proteomic differences. This has been termed the hamburger effect. Little is known about the variability of complex proteomes in response to the environment. Two methods-two-dimensional gel electrophoresis (2DGE) and capillary liquid chromatography-electrospray ionization time-of-flight mass spectrometry (LCMS)-were used to study the hamburger effect in two cross-sections of the soluble fruit fly proteome. 2DGE measured abundant proteins, whereas LCMS measured small proteins and peptides. Proteomic differences between males and females were first evaluated to assess the discriminatory capability of the methods. Likewise, wild-type and white-eyed flies were analyzed as a further demonstration that genetically based proteomic differences could be observed above the background analytical variation. Then dietary interventions were imposed. Ethanol was added to the diet of some populations without significant proteomic effect. However, after a 24-h fast, proteomic differences were found using LCMS but not 2DGE. Even so, only three of approximately 1000 molecular species were altered significantly, suggesting that the influence of even an extreme diet change produced only modest proteomic variability, and that much of the fruit fly proteome remains relatively constant in response to diet. These experiments suggest that proteomics can be a viable approach to biomarker discovery. |
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Authors:
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Thomas F Culwell; Craig D Thulin; Karen J Merrell; Steven W Graves |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biomolecular techniques : JBT Volume: 19 ISSN: 1943-4731 ISO Abbreviation: J Biomol Tech Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2009-01-12 Completed Date: 2009-04-02 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 100888641 Medline TA: J Biomol Tech Country: United States |
Other Details:
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Languages: eng Pagination: 244-50 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biotechnology Chromatography, Liquid Diet* Drosophila melanogaster / metabolism* Electrophoresis, Gel, Two-Dimensional Ethanol / administration & dosage Female Male Proteome / metabolism* Proteomics Spectrometry, Mass, Electrospray Ionization |
| Chemical | |
Reg. No./Substance:
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0/Proteome; 64-17-5/Ethanol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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