| Influence of class B scavenger receptors on cholesterol flux across the brush border membrane and intestinal absorption. | |
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MedLine Citation:
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PMID: 19454765 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To learn more about how the step of cholesterol uptake into the brush border membrane (BBM) of enterocytes influences overall cholesterol absorption, we measured cholesterol absorption 4 and 24 h after administration of an intragastric bolus of radioactive cholesterol in mice with scavenger receptor class B, type 1 (SR-BI) and/or cluster determinant 36 (CD36) deleted. The cholesterol absorption efficiency is unaltered by deletion of either one or both of the receptors. In vitro determinations of the cholesterol uptake specific activity of the BBM from the mice reveal that the scavenger receptors facilitate cholesterol uptake into the proximal BBM. It follows that cholesterol uptake into the BBM is not normally rate-limiting for the cholesterol absorption process in vivo; a subsequent step, such as NPC1L1-mediated transfer from the BBM into the interior of the enterocyte, is rate-limiting. The absorption of dietary cholesterol after 4 h in mice lacking SR-BI and/or CD36 and fed a high-fat/high-cholesterol diet is delayed to more distal regions of the small intestine. This effect probably arises because ATP binding cassette half transporters G5 and G8-mediated back flux of cholesterol from the BBM to the lumen of the small intestine limits absorption and causes the local cholesterol uptake facilitated by SR-BI and CD36 to become rate-limiting under this dietary condition. |
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Authors:
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David V Nguyen; Victor A Drover; Martin Knopfel; Padmaja Dhanasekaran; Helmut Hauser; Michael C Phillips |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-05-19 |
Journal Detail:
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Title: Journal of lipid research Volume: 50 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-12-07 Completed Date: 2010-03-16 Revised Date: 2010-11-02 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 2235-44 Citation Subset: IM |
Affiliation:
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Gastroenterology, Hepatology, and Nutrition Division, Children's Hospital of Philadelphia and University of Pennsylvania School of Medicine, Philadelphia, PA 19104-4318, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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ATP-Binding Cassette Transporters
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genetics Animals Antigens, CD36 / genetics, metabolism Biological Transport Carbon Radioisotopes Cholesterol / metabolism* Enterocytes / cytology, metabolism Gene Expression Intestinal Absorption* Intestine, Small / cytology, metabolism Lipoproteins / genetics Membrane Transport Proteins / genetics Mice Mice, Inbred C57BL Mice, Knockout Microvilli / metabolism* Reverse Transcriptase Polymerase Chain Reaction Scavenger Receptors, Class B / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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HL-22633/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/ABCG5 protein, mouse; 0/ATP-Binding Cassette Transporters; 0/Antigens, CD36; 0/Carbon Radioisotopes; 0/Lipoproteins; 0/Membrane Transport Proteins; 0/Npc1l1 protein, mouse; 0/Scarb1 protein, mouse; 0/Scavenger Receptors, Class B; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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