Document Detail

Influence of cholesterol and ergosterol on membrane dynamics using different fluorescent reporter probes.
MedLine Citation:
PMID:  17374525     Owner:  NLM     Status:  MEDLINE    
Ergosterol is an evolutionary precursor of cholesterol and is the major sterol present in lower eukaryotes. Although detailed biophysical characterization of the effect of cholesterol on membranes is well documented, the effect of ergosterol on the organization and dynamics of membranes is still at a very early stage. We have monitored the effect of cholesterol and ergosterol on the dynamic properties of both fluid (POPC) and gel (DPPC) phase membranes utilizing fluorescent reporter probes pyrene and TMA-DPH. These results show, for the first time, the important differences on the effect of cholesterol and ergosterol in short-range ordering (reported by TMA-DPH) and long-range dynamics (reported by pyrene). In addition, pyrene vibronic peak intensity ratio provides information on polarity of the microenvironment experienced by the probe. These novel results are relevant in the context of membrane domains in ergosterol-containing organisms such as Drosophila which maintain a low level of sterol compared to higher eukaryotes.
Sandeep Shrivastava; Amitabha Chattopadhyay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-12
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  356     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-05     Completed Date:  2007-06-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  705-10     Citation Subset:  IM    
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
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MeSH Terms
1,2-Dipalmitoylphosphatidylcholine / chemistry
Cholesterol / pharmacology*
Diphenylhexatriene / analogs & derivatives
Ergosterol / pharmacology*
Fluorescence Polarization
Fluorescent Dyes
Membranes, Artificial*
Phosphatidylcholines / chemistry
Reg. No./Substance:
0/Fluorescent Dyes; 0/Membranes, Artificial; 0/Phosphatidylcholines; 0/Pyrenes; 129-00-0/pyrene; 1720-32-7/Diphenylhexatriene; 2644-64-6/1,2-Dipalmitoylphosphatidylcholine; 57-87-4/Ergosterol; 57-88-5/Cholesterol; 6753-55-5/1-palmitoyl-2-oleoylphosphatidylcholine; 71316-28-4/1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene

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