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Influence of cellular fatty acid composition on the response of Saccharomyces cerevisiae to hydrostatic pressure stress.
MedLine Citation:
PMID:  22846157     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
High hydrostatic pressure (HHP) interferes with cellular membrane structure. The orientation of lipid molecules is changed, especially in the vicinity of proteins, leading to decreased membrane fluidity. Adaptation to HHP requires increased membrane fluidity, often achieved through a rise in the proportion of unsaturated fatty acids. In this work a desaturase deficient S. cerevisiae mutant strain (OLE1 gene deletion) was grown in media supplemented with fatty acids differing in size and number of unsaturations, and submitted to pressure up to 200 MPa for 30 min. Desaturase-deficient yeast supplemented with palmitoleic acid demonstrated increased sensitivity to pressure compared to cells supplemented with oleic acid or a proportionate mixture of both acids. In contrast, yeast cells grown with linoleic and linolenic acids were more piezoresistant than cells treated with oleic acid. Furthermore, growth with palmitoleic acid led to higher levels of lipid peroxidation. Intracellular trehalose during HHP treatment increased cell tolerance to pressure. However, when trehalose remained extracellular cells were sensitised to pressure. Therefore, fatty acid composition and trehalose content might play a role in the protection of the cell membrane from oxidative damage produced by HHP, confirming that alteration in cell membrane fluidity is correlated with pressure resistance in yeast. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Authors:
Jéssica M de Freitas; Fernanda Bravim; David S Buss; Elenice M Lemos; A Alberto R Fernandes; Patricia M B Fernandes
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-31
Journal Detail:
Title:  FEMS yeast research     Volume:  -     ISSN:  1567-1364     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101085384     Medline TA:  FEMS Yeast Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
Núcleo de Biotecnologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, 29040-090, Brazil.
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