Document Detail

Influence of cell line and cell cycle phase on sonoporation transfection efficiency in cervical carcinoma cells under the same physical conditions.
MedLine Citation:
PMID:  23357919     Owner:  NLM     Status:  In-Data-Review    
Using cervical-carcinoma-derived cells as a model, the present study investigates the effects cell line and cell cycle phase have on sonoporation transfection efficiency under the same physical conditions. A plasmid expressing green fluorescent protein (GFP) was used to measure transfection efficiency. To evaluate the effect of cell type, CaSki, HeLa, and SiHa cells were sonoporated using an acoustic pressure of 1 MPa for 30 s with a duty cycle of 4.8% in the presence of the GFP plasmid. To study the effect of cell cycle phase, SiHa cells were synchronized at S-phase using a double thymidine block and sonoporated at different time points after the block. Contrast agent microbubbles were used at a 0.33% volume concentration. Results indicated that both cell line and cell cycle phase impact the transfection efficiency obtained with sonoporation.
Samuel Pichardo; Melissa Togtema; Robert Jackson; Ingeborg Zehbe; Laura Curiel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  IEEE transactions on ultrasonics, ferroelectrics, and frequency control     Volume:  60     ISSN:  1525-8955     ISO Abbreviation:  IEEE Trans Ultrason Ferroelectr Freq Control     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882735     Medline TA:  IEEE Trans Ultrason Ferroelectr Freq Control     Country:  United States    
Other Details:
Languages:  eng     Pagination:  432-5     Citation Subset:  IM    
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