Document Detail


Influence of biomass and gold salt concentration on nanoparticle synthesis by the tropical marine yeast Yarrowia lipolytica NCIM 3589.
MedLine Citation:
PMID:  19700266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell-associated gold nanoparticles and nanoplates were produced when varying number of Yarrowia lipolytica cells were incubated with different concentrations of chloroauric acid (HAuCl(4)) at pH 4.5. With 10(9)cells ml(-1) and 0.5 or 1.0 mM of the gold salt, the reaction mixtures developed a purple or golden red colour, respectively, and gold nanoparticles were synthesized. Nanoparticles of varying sizes were produced when 10(10)cells ml(-1) were incubated with 0.5, 1.0 or 2.0 mM chloroauric acid salt. With 3.0, 4.0 or 5.0 mM HAuCl(4), nanoplates were also observed. With 10(11)cells ml(-1) nanoparticles were synthesized with almost all the gold salt concentrations. The cell-associated particles were released outside when nanoparticle-loaded cells were incubated at low temperature (20 degrees C) for 48 h. With increasing salt concentrations and a fixed number of cells, the size of the nanoparticles progressively increased. On the other hand, with increasing cell numbers and a constant gold salt concentration, the size of nanoparticles decreased. These results indicate that by varying the number of cells and the gold salt concentration, a variety of nanoparticles and nanoplates can be synthesized. Fourier transform infrared (FTIR) spectroscopy revealed the possible involvement of carboxyl, hydroxyl and amide groups on the cell surfaces in nanoparticle synthesis.
Authors:
P S Pimprikar; S S Joshi; A R Kumar; S S Zinjarde; S K Kulkarni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-07
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  74     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-09-28     Completed Date:  2009-12-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  309-16     Citation Subset:  IM    
Affiliation:
Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, India.
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MeSH Terms
Descriptor/Qualifier:
Biofilms / drug effects,  growth & development
Biomass*
Cell Wall / metabolism
Gold / pharmacology*
Nanoparticles / chemistry,  microbiology*
Particle Size
Seawater / microbiology*
Spectrophotometry, Ultraviolet
Spectroscopy, Fourier Transform Infrared
Tropical Climate*
X-Ray Diffraction
Yarrowia / cytology,  metabolism*,  physiology,  ultrastructure
Chemical
Reg. No./Substance:
7440-57-5/Gold

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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