Document Detail

Influence of backward perfusion on ursodeoxycholate-induced choleresis in isolated in situ rat liver.
MedLine Citation:
PMID:  2254627     Owner:  NLM     Status:  MEDLINE    
Ursodeoxycholate-induced bicarbonate-rich hypercholeresis was studied in isolated in situ forward- or backward-perfused rat livers. Both spontaneous bile flow and bile acid secretion were similar regardless of the direction of the perfusion. The choleretic effect of tauroursodeoxycholate infusion (400 nmol.min-1.100 g-1 body weight) was not significantly different in forward- or backward-perfused livers either. Ursodeoxycholate infusions at low rate (800 nmol.min-1.100 g-1 body weight) induced similar bile flow, bile acid output and bicarbonate output in both forward- and backward-perfused livers. Net ursodeoxycholate uptake, measured as [14C]ursodeoxycholate uptake over the bile acid infusion period (30 min), was not significantly different during forward- or backward-perfusion (4.8 and 5.1 mumol/g liver, respectively); i.e., approx. 67% of infused dose (approximately 7.5 mumol/g liver per 30 min). A 2-fold increase in the dose of ursodeoxycholate infusion (1600 nmol.min-1.100 g-1 b.wt.) induced additional enhancement in both bile flow and bicarbonate biliary secretion, but not in bile acid uptake or output, in forward-perfused livers. Moreover, infusion of the same dose of ursodeoxycholate to backward-perfused livers had a significantly lower choleretic effect (-29%, p less than 0.001) even though ursodeoxycholate uptake and biliary output were similar regardless of perfusion direction. Net ursodeoxycholate uptake, was only 2.4 mumol/g liver; i.e., approx. 16% of infused dose (approximately 15 mumol/g liver per 30 min). These findings indicate that a process related with the hepatic microanatomy may be involved in the hypercholeretic response to ursodeoxycholate.(ABSTRACT TRUNCATED AT 250 WORDS)
F Perez Barriocanal; J J Marin; M Dumont; S Erlinger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of hepatology     Volume:  11     ISSN:  0168-8278     ISO Abbreviation:  J. Hepatol.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1991-01-24     Completed Date:  1991-01-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8503886     Medline TA:  J Hepatol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  165-71     Citation Subset:  IM    
Departamento de Fisiología y Farmacología, Universidad de Salamanca, Spain.
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MeSH Terms
Bicarbonates / metabolism
Bile / secretion*
Liver / drug effects,  secretion*
Perfusion / methods
Rats, Inbred Strains
Taurochenodeoxycholic Acid / adverse effects
Ursodeoxycholic Acid / adverse effects*
Reg. No./Substance:
0/Bicarbonates; 128-13-2/Ursodeoxycholic Acid; 14605-22-2/tauroursodeoxycholic acid; 516-35-8/Taurochenodeoxycholic Acid

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