| Influence of artificial saliva in biofilm formation of Candida albicans in vitro. | |
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MedLine Citation:
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PMID: 22344334 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Due to the increase in life expectancy, new treatments have emerged which, although palliative, provide individuals with a better quality of life. Artificial saliva is a solution that contains substances that moisten a dry mouth, thus mimicking the role of saliva in lubricating the oral cavity and controlling the existing normal oral microbiota. This study aimed to assess the influence of commercially available artificial saliva on biofilm formation by Candida albicans. Artificial saliva I consists of carboxymethylcellulose, while artificial saliva II is composed of glucose oxidase, lactoferrin, lysozyme and lactoperoxidase. A control group used sterile distilled water. Microorganisms from the oral cavity were transferred to Sabouraud Dextrose Agar and incubated at 37 °C for 24 hours. Colonies of Candida albicans were suspended in a sterile solution of NaCl 0.9%, and standardisation of the suspension to 106 cells/mL was achieved. The acrylic discs, immersed in artificial saliva and sterile distilled water, were placed in a 24-well plate containing 2 mL of Sabouraud Dextrose Broth plus 5% sucrose and 0.1 mL aliquot of the Candida albicans suspension. The plates were incubated at 37 °C for 5 days, the discs were washed in 2 mL of 0.9% NaCl and placed into a tube containing 10 mL of 0.9% NaCl. After decimal dilutions, aliquots of 0.1 mL were seeded on Sabouraud Dextrose Agar and incubated at 37 °C for 48 hours. Counts were reported as CFU/mL (Log10). A statistically significant reduction of 29.89% (1.45 CFU/mL) of Candida albicans was observed in saliva I when compared to saliva II (p = 0.002, considering p≤0.05). |
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Authors:
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Michelle Peneluppi Silva; José Chibebe Junior; Adeline Lacerda Jorjão; Ana Karina da Silva Machado; Luciane Dias de Oliveira; Juliana Campos Junqueira; Antonio Olavo Cardoso Jorge |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Brazilian oral research Volume: 26 ISSN: 1807-3107 ISO Abbreviation: Braz Oral Res Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-02-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101307187 Medline TA: Braz Oral Res Country: Brazil |
Other Details:
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Languages: eng Pagination: 24-8 Citation Subset: D; IM |
Affiliation:
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Department of Biosciences and Oral Diagnosis, School of Dentistry of São José dos Campos, Univ. Estadual Paulista, São José dos Campos, SP, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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