Document Detail


Influence of anti-inflammatory and vasoactive drugs on microcirculation and angiogenesis after burn in mice.
MedLine Citation:
PMID:  21334823     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The treatment of burns remains a challenge. Besides the administration of physiological saline, local disinfection and symptomatic medications, no causal therapy is known to accelerate angiogenesis and wound healing. The aim of this study was to investigate the influences of dilatative and anti-inflammatory acting drugs on microcirculation, angiogenesis and leukocyte behavior, which had shown positive effects in former burn studies.
METHODS: The ears of male hairless mice (n=47) were inflicted with full thickness burns using a hot air jet. Then the affects of five intraperitoneal injections of either acetylsalicylic acid (ASA), isosorbide dinitrate, prostaglandin E1 (PGE1) or sodium chloride (each administered to one of four corresponding study groups), on microcirculation, leukocyte-endothelial interaction and angiogenesis were investigated over a 12 day period using intravital fluorescent microscopy.
RESULTS: Angiogenesis was slightly improved by PGE1 (0.3 vs. 1.3% non-perfused area in other groups on day 12, p=0.029). Additionally, blood flow increased and rolling leukocytes decreased compared to other groups. The ASA-group showed best functional vessel density and lowest leukocyte-adhesion. The often described posttraumatic expansion of tissue damage could not be observed in either group.
CONCLUSION: Prostaglandin E1 improved angiogenesis, increased the blood flow and reduced the number of rolling leukocytes. ASA had positive influences on functional vessel density, edema formation and the number of sticking leukocytes. However, it reduced the blood flow. Overall, out of all the drugs tested, prostaglandin seems to have the greatest positive impact on microcirculation and angiogenesis in burns.
Authors:
O Goertz; A Ring; B Buschhaus; T Hirsch; A Daigeler; L Steinstraesser; H-U Steinau; S Langer
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Publication Detail:
Type:  Journal Article     Date:  2011-02-22
Journal Detail:
Title:  Burns : journal of the International Society for Burn Injuries     Volume:  37     ISSN:  1879-1409     ISO Abbreviation:  Burns     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-04-22     Completed Date:  2011-07-22     Revised Date:  2011-10-27    
Medline Journal Info:
Nlm Unique ID:  8913178     Medline TA:  Burns     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  656-64     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd and ISBI. All rights reserved.
Affiliation:
Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr-University Bochum, Bochum, Germany. ole.goertz@rub.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Inflammatory Agents / pharmacology*
Aspirin / analogs & derivatives,  pharmacology
Burns / drug therapy*,  physiopathology
Disease Models, Animal
Endothelium / pathology
Injections, Intraperitoneal
Isosorbide / analogs & derivatives,  pharmacology
Leukocytes / pathology
Mice
Mice, Hairless
Microcirculation / drug effects*
Neovascularization, Pathologic / drug therapy*
Prostaglandins / pharmacology
Skin / blood supply*,  pathology
Sodium Chloride / pharmacology
Vasodilator Agents / pharmacology*
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Prostaglandins; 0/Vasodilator Agents; 0/isosorbide diaspirinate; 50-78-2/Aspirin; 652-67-5/Isosorbide; 7647-14-5/Sodium Chloride
Comments/Corrections
Comment In:
Burns. 2011 Nov;37(7):1272; author reply 1272-3   [PMID:  21821361 ]

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