Document Detail

Influence of amino nitrogen in the culture medium enhances the production of delta-endotoxin and biomass of Bacillus thuringiensis var. israelensis for the large-scale production of the mosquito control agent.
MedLine Citation:
PMID:  18509685     Owner:  NLM     Status:  MEDLINE    
We reported here the role of amino nitrogen in the commercial production of Bacillus thuringiensis var. israelensis media design. The insect pathogen B. thuringiensis var. israelensis was cultured in different media containing varying initial levels of amino nitrogen sources obtained from three different commercial venders. The biomass, mosquito larval toxicity and spore count produced were measured during the fermentation process. The results showed that the higher level of initial amino nitrogen concentrations in the medium led to higher yield of biomass (dry weight 4.78 g l(-1)), larvicidal activity (LC(50) 18.52 ng ml(-1)) and spore count (3.24 x 10(11) CFU ml(-1)). Similarly decreasing the initial amino nitrogen concentration in the medium led to a decreased biomass (dry weight 1.64 g l(-1)), larvicidal activity (LC(50) 27.01 ng ml(-1)) and spore count (3.7 x 10(10) CFUml(-1)).
G Prabakaran; S L Hoti
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Publication Detail:
Type:  Journal Article     Date:  2008-05-29
Journal Detail:
Title:  Journal of industrial microbiology & biotechnology     Volume:  35     ISSN:  1367-5435     ISO Abbreviation:  J. Ind. Microbiol. Biotechnol.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-20     Completed Date:  2008-12-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705544     Medline TA:  J Ind Microbiol Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  961-5     Citation Subset:  IM    
Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry, 605006, India.
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MeSH Terms
Aedes* / drug effects
Bacillus thuringiensis / drug effects*,  growth & development
Bacterial Proteins / biosynthesis*,  toxicity
Culture Media
Endotoxins / biosynthesis*,  toxicity
Hemolysin Proteins / biosynthesis*,  toxicity
Larva / drug effects
Lethal Dose 50
Mosquito Control*
Nitrogen / analysis,  pharmacology*
Time Factors
Reg. No./Substance:
0/Bacterial Proteins; 0/Culture Media; 0/Endotoxins; 0/Hemolysin Proteins; 0/Insecticides; 0/insecticidal crystal protein, Bacillus Thuringiensis; 7727-37-9/Nitrogen

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