Document Detail


Influence of acid stress on survival, expression of virulence genes and invasion capacity into Caco-2 cells of Listeria monocytogenes strains of different origins.
MedLine Citation:
PMID:  19403190     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influence of food-related acid stress on the virulence capacity of Listeria monocytogenes was evaluated. The survival of acid-adapted and non-adapted L. monocytogenes cells during exposure to lethal concentrations of acetic acid was monitored. Also the effect of sublethal acid stress exposure on the expression levels of several virulence genes and on the capacity to invade Caco-2 cells was analyzed. Acid-adapted and non-adapted cells showed different acid tolerance response (ATR) patterns. Our data also demonstrate that pre-exposure to sublethal acid stress might lead to gadD2 induction. However, no correlation with the origin of the strain and with the ATR was noticed, indicating that probably other genes also could play an important role in this ATR mechanism. Additionally, our data showed that acid adaptation could influence the virulence capacity by regulating the expression levels of virulence genes. Moreover, the inlA expression data strongly correlated with the results of the in vitro invasion study. These results lead to the indication that low pH and acetic acid, used in minimally processed food products, might influence the virulence potential of L. monocytogenes.
Authors:
Hadewig Werbrouck; An Vermeulen; Els Van Coillie; Winy Messens; Lieve Herman; Frank Devlieghere; Mieke Uyttendaele
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-05
Journal Detail:
Title:  International journal of food microbiology     Volume:  134     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-24     Completed Date:  2009-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  140-6     Citation Subset:  IM    
Affiliation:
Flemish Government, Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium. Hadewig.Werbrouck@ilvo.vlaanderen.be
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological*
Bacterial Adhesion / physiology
Caco-2 Cells
Colony Count, Microbial
Consumer Product Safety*
Food Microbiology*
Gene Expression Regulation, Bacterial / genetics*
Humans
Hydrogen-Ion Concentration
Kinetics
Listeria monocytogenes* / genetics,  pathogenicity,  physiology
Virulence / genetics

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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