| Influence of Tumor associated Macrophages on Biological Function of SW620 Cell. | |
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MedLine Citation:
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PMID: 21375942 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objective To study the influence of tumor-associated macrophages (TAMs) on the biological function of SW620 cell. Methods Macrophage was induced into M2-type macrophage form with interleukin (IL)-4. CD68, macrophage mannose receptor (MMR), and inducible nitric oxide synthase (iNOS) were analyzed with Western blot. SW620 was co-cultured with TAMs in the Transwell. Cytokines including IL-10, IL-12, IL-23, and tramsforming growth factor-β(TGF-β) were detected with enzyme-linked immunosorbent assay (ELISA). The activity of nuclear factor-κB (NF-κB) in SW620 was analyzed with electrophoretic mobility shift assay (EMSA). The proliferation and apoptosis of SW620 cells after co-cultured with TAM were determined with tetrazolium four nitrogen (XTT) assay and fluorescence activated cell sorting (FACS), respectively. Results IL-4 induced M2 type macrophage expressed CD68 and MMR instead of iNOS. After co-cultured with SW620 for 24 hours and 48 hours, M2 type macrophage secreted higher levels of IL-10 and TGF-βthan the pre-culture level (P0.05). The activity of NF-κB in SW620 decreased by 72% and 75% after 24 hours and 48 hours compared with the pre-culture level, respectively (both P<0.01). The activity of proliferation decreased by 48% and 59% and the apoptotic rates increased by 6.37% and 7.68% and 0.37% after 24 hours and 48 hours (all P<0.01) compared with the pre-culture levels. Conclusion TAM may inhibit the proliferation and promote the apoptosis of SW620 by suppressing the activity of NF-κB. |
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Authors:
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Zhang Yin-Xu; Liu Xiao Mei; Zuo Teng; Liu Yu; Zhang Jun Hua |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae Volume: 33 ISSN: 1000-503X ISO Abbreviation: Zhongguo Yi Xue Ke Xue Yuan Xue Bao Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-03-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8006230 Medline TA: Zhongguo Yi Xue Ke Xue Yuan Xue Bao Country: China |
Other Details:
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Languages: eng Pagination: 71-5 Citation Subset: IM |
Affiliation:
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Department of General Surgery,the First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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