Document Detail


The influence of therapeutic radiation on the patterns of bone remodeling in ovary-intact and ovariectomized mice.
MedLine Citation:
PMID:  23314741     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our purpose was to characterize changes in bone remodeling associated with localized radiation that models therapeutic cancer treatment in ovary-intact (I) and ovariectomized (OVX) mice and to evaluate the influence of radiation on the pattern of bone mineral remodeling. Young adult, female BALB/c mice, I and OVX, were used (n = 71). All mice were intravenously injected with 15 μCi (45)Ca. Thirty days post-(45)Ca administration, the hind limbs of 17 mice were exposed to a single dose of 16 Gy radiation (R). The time course of (45)Ca excretion, serum CTx and osteocalcin markers, and cancellous bone volume fraction (BV/TV) and cortical thickness (Ct.Th) of the distal femur were assayed. Cellular activity and dynamic histomorphometry were performed. Irradiation resulted in rapid increases in fecal (45)Ca excretion compared to control groups, indicating increased bone remodeling. CTx increased rapidly after irradiation, followed by an increase in osteocalcin concentration. BV/TV decreased in the I mice following irradiation. Ct.Th increased in the OVX groups following irradiation. I+R mice exhibited diminished osteoblast surface, osteoclast number, and mineral apposition. Our murine model showed the systemic effects (via (45)Ca excretion) and local effects (via bone microarchitecture and surface activity) of clinically relevant, therapeutic radiation exposure. The I and OVX murine models have similar (45)Ca excretion but different bone microarchitectural responses. The (45)Ca assay effectively indicates the onset and rate of systemic bone mineral remodeling, providing real-time assessment of changes in bone histomorphometric parameters. Monitoring bone health via a bone mineral marker may help to identify the appropriate time for clinical intervention to preserve skeletal integrity.
Authors:
Susanta K Hui; Gregory R Fairchild; Louis S Kidder; Manju Sharma; Maryka Bhattacharya; Scott Jackson; Chap Le; Anna Petryk; Mohammad Saiful Islam; Douglas Yee
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-12
Journal Detail:
Title:  Calcified tissue international     Volume:  92     ISSN:  1432-0827     ISO Abbreviation:  Calcif. Tissue Int.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-11     Completed Date:  2013-09-16     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  7905481     Medline TA:  Calcif Tissue Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  372-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Bone Remodeling / physiology,  radiation effects*
Bone and Bones / metabolism*,  radiation effects*,  radiography
Calcium Radioisotopes / metabolism
Collagen Type I / metabolism
Dose-Response Relationship, Radiation
Female
Mice
Mice, Inbred BALB C
Models, Animal
Osteocalcin / metabolism
Ovariectomy*
Ovary / physiology,  surgery*
Peptides / metabolism
Radiotherapy*
Time Factors
X-Ray Microtomography
Grant Support
ID/Acronym/Agency:
1K12-HD055887-01/HD/NICHD NIH HHS; 1R03AR055333-01A1/AR/NIAMS NIH HHS; P30 CA77398/CA/NCI NIH HHS; R01 CA154491/CA/NCI NIH HHS; R03 AR055333/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Calcium Radioisotopes; 0/Collagen Type I; 0/Peptides; 0/collagen type I trimeric cross-linked peptide; 104982-03-8/Osteocalcin
Comments/Corrections

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