| Influence of Temperature, Hypercapnia, and Development on the Relative Expression of Different Hemocyanin Isoforms in the Common Cuttlefish Sepia officinalis. | |
| | |
MedLine Citation:
|
PMID: 22791630 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The cuttlefish Sepia officinalis expresses several hemocyanin isoforms with potentially different pH optima, indicating their reliance on efficient pH regulation in the blood. Ongoing ocean warming and acidification could influence the oxygen-binding properties of respiratory pigments in ectothermic marine invertebrates. This study examined whether S. officinalis differentially expresses individual hemocyanin isoforms to maintain optimal oxygen transport during development and acclimation to elevated seawater pCO(2) and temperature. Using quantitative PCR, we measured relative mRNA expression levels of three different hemocyanin isoforms in several ontogenetic stages (embryos, hatchlings, juveniles, and adults), under different temperatures and elevated seawater pCO(2) . Our results indicate moderately altered hemocyanin expression in all embryonic stages acclimated to higher pCO(2) , while hemocyanin expression in hatchlings and juveniles remained unaffected. During the course of development, total hemocyanin expression increased independently of pCO(2) or thermal acclimation status. Expression of isoform 3 is reported for the first time in a cephalopod in this study and was found to be generally low but highest in the embryonic stages (0.2% of total expression). Despite variable hemocyanin expression, hemolymph total protein concentrations remained constant in the experimental groups. Our data provide first evidence that ontogeny has a stronger influence on hemocyanin isoform expression than the environmental conditions chosen, and they suggest that hemocyanin protein abundance in response to thermal acclimation is regulated by post-transcriptional/translational rather than by transcriptional modifications. J. Exp. Zool. 9999A:000-000, 2012. © 2012 Wiley Periodicals, Inc. |
| | |
Authors:
|
Anneli Strobel; Marian Y A Hu; Magdalena A Gutowska; Bernhard Lieb; Magnus Lucassen; Frank Melzner; Hans O Pörtner; Felix C Mark |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-7-12 |
Journal Detail:
|
Title: Journal of experimental zoology. Part A, Ecological genetics and physiology Volume: 9999 ISSN: 1932-5231 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
|
Created Date: 2012-7-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101297745 Medline TA: J Exp Zool A Ecol Genet Physiol Country: - |
Other Details:
|
Languages: ENG Pagination: 1-13 Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
|
Integrative Ecophysiology, Alfred Wegener Institute for Polar and Marine Research, Bremerhaven, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A cell population that strongly expresses the CB1 cannabinoid receptor in the central canal of the r...
Next Document: The development of boronic acids as sensors and separation tools.