| Influence of salt concentration on the assembly of DNA multilayer films. | |
| | |
MedLine Citation:
|
PMID: 19891451 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
DNA multilayer films are promising candidates for a plethora of applications, including sensing, diagnostics, and drug/gene delivery. Fabricated solely from DNA, the use of salt in forming DNA multilayers is crucial in promoting and maintaining hybridization of complementary base pairs by minimizing the repulsive forces between the oligonucleotides and preventing disassembly of the layers once formed. Herein, we examine the role of salt on the assembly of DNA films assembled from oligonucleotides composed of two homopolymeric diblocks (polyA(n)G(n) and polyT(n)C(n)) in salt concentrations ranging from 0.1 to 2 M. Using quartz crystal microgravimetry (QCM) and flow cytometry, we show that films assembled at high salt concentrations (2 M salt) exhibit a different morphology and are denser than those assembled from lower (1 M salt) salt solutions. Formation of the T x A*T triplex in solution and within the DNA film was also studied using circular dichroism (CD) and QCM, respectively. DNA films assembled using oligonucleotides of various lengths (20- to 60-mer) at high salt concentration (2 M salt) showed no significant influence on the film growth. This work shows that salt plays an important role in the assembly and final morphology of DNA multilayer films, hence enabling films with different properties to be tailored. |
| | |
Authors:
|
Lillian Lee; Francesca Cavalieri; Angus P R Johnston; Frank Caruso |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Langmuir : the ACS journal of surfaces and colloids Volume: 26 ISSN: 1520-5827 ISO Abbreviation: Langmuir Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-02-23 Completed Date: 2010-05-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9882736 Medline TA: Langmuir Country: United States |
Other Details:
|
Languages: eng Pagination: 3415-22 Citation Subset: IM |
Affiliation:
|
Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Victoria 3010, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Circular Dichroism Colloids DNA / chemistry* Elasticity Poly A / chemistry Poly T / chemistry Quartz Salts / chemistry* Solutions Spectrometry, Fluorescence Viscosity |
| Chemical | |
Reg. No./Substance:
|
0/Colloids; 0/Salts; 0/Solutions; 0/triplex DNA; 14808-60-7/Quartz; 24937-83-5/Poly A; 25086-81-1/Poly T; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Formation of Rh-Au Core-Shell Nanoparticles on TiO(2)(110) Surface Studied by STM and LEIS.
Next Document: Differential Magnetic Catch and Release: Analysis and Separation of Magnetic Nanoparticles.