Document Detail


Influence of Q- and G-banding on the Feulgen-stainability of human metaphase chromosomes.
MedLine Citation:
PMID:  63454     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper, model experiments on chicken red blood cell nuclei are described concerning the influence of methanol-acetic acid fixation and irradiation at different wavelengths, with and without prior Atebrin staining on subsequent Feulgen-stainability. In addition, data are reported on the influence on Feulgen-stainability of Giemsa-banding procedures, illumination of unstained chromosomes at 220 and 515 nm and exposure of unstained and Atebrin-stained chromosomes to illumination at 440 nm. The ASG and especially the trypsin-Giemsa technique appeared to reduce markedly Feulgen-stainability. The same holds true for Atebrin fluorescence of chromosomes. The data are discussed in relation to their implications for the assumed cause of the Q- and G-banding phenomena. Techniques are described that allow reliable Feulgen DNA measurements of individual chromosomes after application of either G- or Q-banding.
Authors:
F T Bosman; M Van Der Ploeg; J P Geraedts
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Histochemical journal     Volume:  9     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:  1977 Jan 
Date Detail:
Created Date:  1977-02-24     Completed Date:  1977-02-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  31-42     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Azure Stains / diagnostic use
Chickens
Chromosomes / analysis*,  radiation effects
DNA / analysis*
Fixatives
Humans
Light
Mitosis
Quinacrine Mustard
Staining and Labeling*
Ultraviolet Rays
Chemical
Reg. No./Substance:
0/Azure Stains; 0/Fixatives; 4213-45-0/Quinacrine Mustard; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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