Document Detail

Influence of lineage-specific amino acid dimorphisms in GyrA on Mycobacterium tuberculosis resistance to fluoroquinolones.
MedLine Citation:
PMID:  22274162     Owner:  NLM     Status:  MEDLINE    
We conducted in vitro DNA supercoiling assays, utilizing recombinant DNA gyrases, to elucidate the influence of the lineage-specific serine or threonine residue at position 95 of GyrA on fluoroquinolone resistance in Mycobacterium tuberculosis. There was little effect of the GyrA-Ala74Ser amino acid substitution on activity of the GyrA-Ser95 gyrase, while activity of the GyrA-Asp94Gly-Ser95 gyrase was reduced. These findings were in striking contrast to previous reports analyzing GyrA with Thr95 and suggest an important impact of the amino acid in the development of fluoroquinolone resistance.
Hyun Kim; Chie Nakajima; Youn Uck Kim; Kazumasa Yokoyama; Yasuhiko Suzuki
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Japanese journal of infectious diseases     Volume:  65     ISSN:  1884-2836     ISO Abbreviation:  Jpn. J. Infect. Dis.     Publication Date:  2012  
Date Detail:
Created Date:  2012-01-25     Completed Date:  2012-05-17     Revised Date:  2012-07-16    
Medline Journal Info:
Nlm Unique ID:  100893704     Medline TA:  Jpn J Infect Dis     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  72-4     Citation Subset:  IM    
Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan.
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MeSH Terms
Amino Acid Substitution*
DNA Gyrase / genetics,  metabolism*
DNA, Bacterial / genetics
DNA, Superhelical / genetics
Drug Resistance, Bacterial
Enzyme Activation
Escherichia coli / genetics,  metabolism
Fluoroquinolones / pharmacology*
Microbial Sensitivity Tests
Mycobacterium tuberculosis / drug effects*,  enzymology*,  genetics
Plasmids / genetics,  metabolism
Recombinant Proteins / genetics,  metabolism
Serine / genetics,  metabolism
Threonine / genetics,  metabolism
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Superhelical; 0/Fluoroquinolones; 0/Recombinant Proteins; 56-45-1/Serine; 72-19-5/Threonine; EC 5.99.1.-/DNA Gyrase

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