Document Detail


The influence of lamellar orientation on corneal material behavior: biomechanical and structural changes in an avian corneal disorder.
MedLine Citation:
PMID:  21051696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Retinopathy, globe enlarged (RGE) is an inherited genetic disease of chickens with a corneal phenotype characterized by loss of tissue curvature and changes in peripheral collagen fibril alignment. This study aimed to characterize the material behavior of normal and RGE chicken corneas under inflation and compare this with new spatial- and depth-resolved microstructural information to investigate how stromal fibril architecture determines corneal behavior under intraocular pressure (IOP).
METHODS: Six RGE chicken corneas and six age-matched normal controls were tested using trephinate inflation and their stress-strain behavior determined as a function of posterior pressure. Second harmonic generation mulitphoton microscopy was used to compare the in-plane appearance and degree of through-plane interlacing of collagen lamellae between normal and mutant corneas.
RESULTS: RGE corneas displayed a 30-130% increase in material stiffness [E(tangent)(RGE) = 0.94 ± 0.18 MPa to 3.09 ± 0.66 MPa; E(tangent)(normals) = 0.72 ± 0.13 MPa to 1.34 ± 0.35 MPa] (P ≤ 0.05). The normal in-plane disposition of anterior collagen in the peripheral cornea was altered in RGE but through-plane lamellar interlacing was unaffected.
CONCLUSIONS: This article demonstrates changes in corneal material behavior in RGE that are qualitatively consistent with microstructural collagen alterations identified both herein and previously. This study indicates that, in general, changes in stromal fibril orientation may significantly affect corneal material behavior and thereby its response to IOP.
Authors:
Craig Boote; Ahmed Elsheikh; Wael Kassem; Christina S Kamma-Lorger; Paul M Hocking; Nick White; Chris F Inglehearn; Manir Ali; Keith M Meek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-03-10
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  52     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-14     Completed Date:  2011-05-20     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1243-51     Citation Subset:  IM    
Affiliation:
Structural Biophysics Group, School of Optometry and Vision Sciences, Cardiff University, Cardiff, United Kingdom. bootec@cf.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomechanics / physiology*
Blindness / genetics,  pathology,  veterinary*
Chickens*
Collagen / metabolism*
Corneal Diseases / genetics,  metabolism,  pathology,  veterinary*
Corneal Stroma / metabolism*,  physiopathology
Elastic Tissue
Hypertrophy
Intraocular Pressure
Microscopy, Fluorescence, Multiphoton
Poultry Diseases / genetics,  metabolism*,  pathology
Retinal Degeneration / genetics,  pathology,  veterinary*
Grant Support
ID/Acronym/Agency:
074165//Wellcome Trust; G0501050//Medical Research Council; G0600755//Medical Research Council; //Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
9007-34-5/Collagen
Comments/Corrections

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