| Influence of Lactic Acid on Endogenous and Viral RNA-Induced Immune Mediator Production by Vaginal Epithelial Cells. | |
| | |
MedLine Citation:
|
PMID: 21934447 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
OBJECTIVE: : To evaluate the influence of lactic acid on immune mediator release from vaginal epithelial cells. METHODS: : The human vaginal epithelial cell line, VK2/E6E7, was cultured in the presence or absence of physiological concentrations of lactic acid, and in the presence or absence of the viral Toll-like receptor 3 agonist, poly (inosinic acid:cytidylic acid). Supernatants were assayed by enzyme-linked immunosorbent assay (ELISA) for interleukin (IL)-1β, IL-6, IL-8, IL-23, transforming growth factor (TGF)-β and secretory leukocyte protease inhibitor. RESULTS: : Vaginal epithelial cells spontaneously released IL-1β (25.9 pg/mL), IL-8 (1.0 ng/mL), TGF-β (175 pg/mL), and secretory leukocyte protease inhibitor (33.8 ng/mL). Only TGF-β production was marginally enhanced (49%) by addition of lactic acid alone. Poly (inosinic acid:cytidylic acid) by itself stimulated the release of IL-6 (305 pg/mL) and enhanced IL-8 production (2.8 ng/mL). The combination of poly (inosinic acid:cytidylic acid) and lactic acid markedly increased IL-8 production (5.0 ng/mL) and induced the release of IL-1β (96.2 pg/mL). The poly (inosinic acid:cytidylic acid)-mediated lactic acid effect on IL-1β and IL-8 release was abrogated when the lactic acid was neutralized or if acetic acid was substituted for lactic acid. CONCLUSION: : Lactic acid enhances the release of selective mediators from vaginal epithelial cells and stimulates antiviral immune responses. |
| | |
Authors:
|
Hannah Mossop; Iara M Linhares; Ann Marie Bongiovanni; William J Ledger; Steven S Witkin |
Related Documents
:
|
7164697 - Studies on lectins. lii. isolation and characterization of the lectin from rye germ (se... 1427977 - Interleukin-1 receptor antagonist in inflammatory exudate cells of rabbits. production,... 3938267 - P-cresol formation by cell-free extracts of clostridium difficile. 19871947 - Banana gel. 10228767 - Bile acids as components of the duodenogastric refluxate: detection, relationship to bi... 22507387 - Kujigamberol, a new dinorlabdane diterpenoid isolated from 85ma old kuji amber using a ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Obstetrics and gynecology Volume: 118 ISSN: 1873-233X ISO Abbreviation: Obstet Gynecol Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-09-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0401101 Medline TA: Obstet Gynecol Country: United States |
Other Details:
|
Languages: eng Pagination: 840-6 Citation Subset: AIM; IM |
Affiliation:
|
From the Division of Immunology and Infectious Diseases, Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, New York; and the Department of Gynecology and Obstetrics, University of Sao Paulo Medical School and Hospital das Clinicas, Sao Paulo, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genital Warts and Vulvar Intraepithelial Neoplasia: Natural History and Effects of Treatment and Hum...
Next Document: Clinical features of early-stage nonhydropic mole for diagnosis of persistent trophoblastic disease.