Document Detail


Influence of LDL oxidation on the proliferation of human breast cancer cells.
MedLine Citation:
PMID:  8903687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of lipid peroxidation and the antioxidant vitamin E contained in LDL isolated from control plasma (LDL--) and from plasma preincubated with 0.5 mmol/ml alpha-tocopherol (LDL+) on the proliferation of estrogen-receptor positive (ER+ : ZR-75, T-47-D, MCF-7) and negative (ER--: HBL-100, MDA-MB-231) human breast cancer cells were studied. Human skin fibroblasts served as controls. Incubation of plasma with 0.5 mmol/ml alpha-tocopherol resulted in a 3-fold increase of its content and a significant reduction in lipid hydroperoxides and conjugated dienes in LDL. Incubation of fibroblasts or ER+ tumor cells with LDL- or LDL+ had an effect on neither cell proliferation nor on the cellular levels of peroxidation products as compared to control incubations in the absence of LDL. In ER- cells, however, LDL+ stimulated the proliferation, whereas LDL- yielded a cytotoxic effect. Moreover, LDL- supplementation resulted in an increase in the content of hydroperoxides and conjugated dienes. LDL+ supplemented cells exhibited hydroperoxide levels in these tumor cells comparable to the basal levels measured in the absence of LDL. Our data suggested that peroxidation products in LDL are cytotoxic to estrogen-receptor negative breast tumor cells and vitamin E counteracts this effect.
Authors:
V Chajes; M Mahon; G M Kostner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical biology & medicine     Volume:  20     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  1996  
Date Detail:
Created Date:  1997-03-14     Completed Date:  1997-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  113-20     Citation Subset:  IM    
Affiliation:
Institute of Medical Biochemistry, University of Graz, Austria.
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / metabolism
Cell Division* / drug effects
Cell Survival
Fibroblasts
Humans
Lipid Peroxidation / physiology
Lipid Peroxides / metabolism,  toxicity
Lipoproteins, LDL / pharmacology*
Receptors, Estrogen / metabolism
Tumor Cells, Cultured
Vitamin E / pharmacology
Chemical
Reg. No./Substance:
0/Lipid Peroxides; 0/Lipoproteins, LDL; 0/Receptors, Estrogen; 1406-18-4/Vitamin E

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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