| Influence of LDL oxidation on the proliferation of human breast cancer cells. | |
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MedLine Citation:
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PMID: 8903687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of lipid peroxidation and the antioxidant vitamin E contained in LDL isolated from control plasma (LDL--) and from plasma preincubated with 0.5 mmol/ml alpha-tocopherol (LDL+) on the proliferation of estrogen-receptor positive (ER+ : ZR-75, T-47-D, MCF-7) and negative (ER--: HBL-100, MDA-MB-231) human breast cancer cells were studied. Human skin fibroblasts served as controls. Incubation of plasma with 0.5 mmol/ml alpha-tocopherol resulted in a 3-fold increase of its content and a significant reduction in lipid hydroperoxides and conjugated dienes in LDL. Incubation of fibroblasts or ER+ tumor cells with LDL- or LDL+ had an effect on neither cell proliferation nor on the cellular levels of peroxidation products as compared to control incubations in the absence of LDL. In ER- cells, however, LDL+ stimulated the proliferation, whereas LDL- yielded a cytotoxic effect. Moreover, LDL- supplementation resulted in an increase in the content of hydroperoxides and conjugated dienes. LDL+ supplemented cells exhibited hydroperoxide levels in these tumor cells comparable to the basal levels measured in the absence of LDL. Our data suggested that peroxidation products in LDL are cytotoxic to estrogen-receptor negative breast tumor cells and vitamin E counteracts this effect. |
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Authors:
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V Chajes; M Mahon; G M Kostner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Free radical biology & medicine Volume: 20 ISSN: 0891-5849 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 1996 |
Date Detail:
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Created Date: 1997-03-14 Completed Date: 1997-03-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 113-20 Citation Subset: IM |
Affiliation:
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Institute of Medical Biochemistry, University of Graz, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Breast Neoplasms
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metabolism Cell Division* / drug effects Cell Survival Fibroblasts Humans Lipid Peroxidation / physiology Lipid Peroxides / metabolism, toxicity Lipoproteins, LDL / pharmacology* Receptors, Estrogen / metabolism Tumor Cells, Cultured Vitamin E / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Lipid Peroxides; 0/Lipoproteins, LDL; 0/Receptors, Estrogen; 1406-18-4/Vitamin E |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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