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Influence of Glutathione on the Electroretinogram in Diabetic and Non-diabetic Rats.
MedLine Citation:
PMID:  21851169     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Aims: The purpose of this study was to investigate the influence of glutathione on the electroretinogram (ERG) in diabetic and non-diabetic rats. Materials and Methods: Streptozotocin (STZ: 60 mg/kg) was injected into male RCC Wistar rats to induce hyperglycemia, with buffer instead of STZ injected into age-matched non-diabetic controls. After 8 weeks, ERG measurements were obtained at seven different scotopic flash intensities on the two groups of anesthetized, dark-adapted rats (controls, STZ). Following ERG measurements, eyes were enucleated for measurements of retinal/vitreous GSH and glutathione disulfide (GSSG). Results: Diabetic rats produced delayed b-wave ERG signals (increased implicit times), but had normal a-wave and b-wave amplitudes, a-wave implicit times, and oscillatory potentials. No differences were observed in retinal GSH or GSSG between controls and diabetics; however, correlations between GSH and all ERG parameters (with the exception of b-wave implicit times) were noted, and were not significantly altered by the presence of hyperglycemia. Conclusions: GSH is likely to play an important role in retinal function as assessed by the ERG, with this role not substantially altered in rats diabetic for 8 weeks.
Authors:
William S Wright; Robert M McElhatten; Carmina Busu; Singh Yadav Amit; Wendy Leskova; Tak Yee Aw; Norman R Harris
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current eye research     Volume:  36     ISSN:  1460-2202     ISO Abbreviation:  Curr. Eye Res.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8104312     Medline TA:  Curr Eye Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  831-7     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center , Shreveport, Louisiana , USA.
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