| Influence of estrogen and polyamines on mifepristone-induced apoptosis in prostate cancer cells. | |
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MedLine Citation:
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PMID: 20396571 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Although androgens are the main steroids controlling the growth of prostate glands, estrogens are also important in the regulation of its growth. Prostate cancer cells, like other cancer cells, maintain high levels of polyamines. In LNCaP cells, apoptosis is induced by mifepristone. During the process of cell death, the regulation of ROS production, caspase-3 activation and poly (ADP-ribose) polymerase cleavage were investigated in the presence of estrogen and polyamines to identify their possible roles. MATERIALS AND METHODS: The cell growth was assessed using the MTT assay, and the intracellular ROS production by the DCFH-DA assay. The p53 protein expression, activation of caspase-3 and PARP cleavage were checked by Western blotting, with specific antibodies to each. RESULTS: The growth and viability of the cells were significantly inhibited, in a dose- and time-dependent manners, by mifepristone (MIF) treatment. The production of ROS were dependent on the MIF dosage. The activation of caspase-3 and cleavage of PARPalso increased with the duration of MIF treatment. The expression of p53 protein also increased with increases in the MIF incubation time. E(2) severely inhibited the ROS production, caspase-3 activation and PARP cleavage. However, polyamines only inhibited the ROS production, without influencing the caspase-3 activation or PARP cleavage. CONCLUSION: In LNCaP cells, MIF induces apoptosis through ROS production. The expression of p53 protein, caspase-3 activation and PARP cleavage accompanied the process of apoptosis. The apoptotic processes were inhibited by E(2), but polyamines only inhibited the ROS production, implying the multifunctional role of E(2), in addition to its role as a free radical scavenger. |
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Authors:
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Eun Kyung Choi; Hwi-June Song; Min S Park; Byeong Gee Kim |
Publication Detail:
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Type: Journal Article Date: 2004-02-29 |
Journal Detail:
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Title: Cancer research and treatment : official journal of Korean Cancer Association Volume: 36 ISSN: 2005-9256 ISO Abbreviation: Cancer Res Treat Publication Date: 2004 Feb |
Date Detail:
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Created Date: 2010-04-16 Completed Date: 2010-06-09 Revised Date: 2010-09-30 |
Medline Journal Info:
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Nlm Unique ID: 101155137 Medline TA: Cancer Res Treat Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 85-90 Citation Subset: - |
Affiliation:
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Department of Biology, Pusan National University, Busan, Korea. |
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