| The influence of electric fields on hippocampal neural progenitor cells. | |
| | |
MedLine Citation:
|
PMID: 20665129 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The differentiation and proliferation of neural stem/progenitor cells (NPCs) depend on various in vivo environmental factors or cues, which may include an endogenous electrical field (EF), as observed during nervous system development and repair. In this study, we investigate the morphologic, phenotypic, and mitotic alterations of adult hippocampal NPCs that occur when exposed to two EFs of estimated endogenous strengths. NPCs treated with a 437 mV/mm direct current (DC) EF aligned perpendicularly to the EF vector and had a greater tendency to differentiate into neurons, but not into oligodendrocytes or astrocytes, compared to controls. Furthermore, NPC process growth was promoted perpendicularly and inhibited anodally in the 437 mV/mm DC EF. Yet fewer cells were observed in the DC EF, which in part was due to a decrease in cell viability. The other EF applied was a 46 mV/mm alternating current (AC) EF. However, the 46 mV/mm AC EF showed no major differences in alignment or differentiation, compared to control conditions. For both EF treatments, the percent of mitotic cells during the last 14 h of the experiment were statistically similar to controls. Reported here, to our knowledge, is the first evidence of adult NPC differentiation affected in an EF in vitro. Further investigation and application of EFs on stem cells is warranted to elucidate the utility of EFs to control phenotypic behavior. With progress, the use of EFs may be engineered to control differentiation and target the growth of transplanted cells in a stem cell-based therapy to treat nervous system disorders. |
| | |
Authors:
|
Carlos Atico Ariza; Asha T Fleury; Christian J Tormos; Vadim Petruk; Sagar Chawla; Jisun Oh; Donald S Sakaguchi; Surya K Mallapragada |
Related Documents
:
|
19330499 - Neurological disorders and neural regeneration, with special reference to parkinson's d... 16390999 - Embryonic stem cells assume a primitive neural stem cell fate in the absence of extrins... 17280659 - Transient inactivation of notch signaling synchronizes differentiation of neural progen... 17597119 - Phenotypic characterization of neural stem cells from human fetal spinal cord: synergis... 16894609 - Endogenous bone morphogenetic protein antagonists regulate mammalian neural crest gener... 16878319 - Function of id1 protein in human cord blood-derived neural stem-like cells. 22070799 - Bystander activation of inkt cells occurs during conventional t-cell alloresponses. 1830599 - Beta 1 integrin (cd29) expression on human postnatal t cell subsets defined by selectiv... 21552419 - Novel therapeutic strategies for targeting liver cancer stem cells. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Stem cell reviews Volume: 6 ISSN: 1558-6804 ISO Abbreviation: Stem Cell Rev Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-10-04 Completed Date: 2011-01-20 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101255952 Medline TA: Stem Cell Rev Country: United States |
Other Details:
|
Languages: eng Pagination: 585-600 Citation Subset: IM |
Affiliation:
|
Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Count Cell Movement / physiology Cell Proliferation Electricity* Hippocampus / cytology* Immunohistochemistry Neurons / cytology* Rats Rats, Inbred F344 Stem Cells / cytology* |
| Grant Support | |
ID/Acronym/Agency:
|
1 R01 GM072005/GM/NIGMS NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A consensus statement addressing mesenchymal stem cell transplantation for multiple sclerosis: it's ...
Next Document: Prevalence and diagnosis of primary aldosteronism.