| Influence of cysteamine on in vitro maturation, in vitro and in vivo fertilization of equine oocytes. | |
| | |
MedLine Citation:
|
PMID: 18992121 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Contents The effect of cysteamine on in vitro nuclear and cytoplasmic maturation of equine oocytes collected by transvaginal ultrasound guided follicular aspiration was assessed. Oocytes were matured in vitro with (cysteamine group) or without (control group) cysteamine. The nuclear stage after DNA Hoechst staining, penetration rates after two different in vitro fertilization (IVF) techniques (IVF media with ionophore and Hepes buffer with heparin) and the embryo yield following oocyte intra-oviductal transfer were used as a criterion for assessing nuclear and cytoplasmic maturation, respectively. Contrary to the data described in other domestic species, there was no effect of cysteamine on in vitro nuclear maturation, IVF or in vivo embryonic development under our conditions. Ovum pick up yields (52%) and maturation rates (control group: 47% and cysteamine group: 55%) were similar to those previously reported. From 57 oocytes transferred to the oviduct in each group, the number of embryos collected was 10 (17%) in the control group and five in the cysteamine group (9%). Those two percentages were not statistically different (p > 0.05). No effect of IVF technique was seen on the success rate (6%) in each group. |
| | |
Authors:
|
S Deleuze; C S Dubois; M Caillaud; B Bruneau; G Goudet; G Duchamp |
Publication Detail:
|
Type: Journal Article Date: 2008-10-28 |
Journal Detail:
|
Title: Reproduction in domestic animals = Zuchthygiene Volume: 45 ISSN: 1439-0531 ISO Abbreviation: Reprod. Domest. Anim. Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2010-02-08 Completed Date: 2010-04-26 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9015668 Medline TA: Reprod Domest Anim Country: Germany |
Other Details:
|
Languages: eng Pagination: 1-7 Citation Subset: IM |
Affiliation:
|
Facult? de M?decine V?t?rinaire, D?partement des Sciences Cliniques-Clinique Equine, Universit? de Li?ge, Li?ge, Belgium. s.deleuze@ulg.ac.be |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Nucleus / drug effects, physiology Cells, Cultured Cysteamine / pharmacology* Cytoplasm / drug effects, physiology Embryo, Mammalian / physiology Fallopian Tubes Female Fertilization / drug effects* Fertilization in Vitro / drug effects, veterinary* Horses* Insemination, Artificial / veterinary Male Oocytes / drug effects*, transplantation, ultrastructure Spermatozoa / physiology Tissue and Organ Harvesting / methods, veterinary |
| Chemical | |
Reg. No./Substance:
|
60-23-1/Cysteamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Number of spermatozoa in the crypts of the sperm reservoir at about 24 h after a low-dose intrauteri...
Next Document: Evaluation of Efficacy and Safety of Zinc Gluconate Associated with Dimethyl Sulphoxide for Sexually...