Document Detail


Influence of cysteamine on in vitro maturation, in vitro and in vivo fertilization of equine oocytes.
MedLine Citation:
PMID:  18992121     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Contents The effect of cysteamine on in vitro nuclear and cytoplasmic maturation of equine oocytes collected by transvaginal ultrasound guided follicular aspiration was assessed. Oocytes were matured in vitro with (cysteamine group) or without (control group) cysteamine. The nuclear stage after DNA Hoechst staining, penetration rates after two different in vitro fertilization (IVF) techniques (IVF media with ionophore and Hepes buffer with heparin) and the embryo yield following oocyte intra-oviductal transfer were used as a criterion for assessing nuclear and cytoplasmic maturation, respectively. Contrary to the data described in other domestic species, there was no effect of cysteamine on in vitro nuclear maturation, IVF or in vivo embryonic development under our conditions. Ovum pick up yields (52%) and maturation rates (control group: 47% and cysteamine group: 55%) were similar to those previously reported. From 57 oocytes transferred to the oviduct in each group, the number of embryos collected was 10 (17%) in the control group and five in the cysteamine group (9%). Those two percentages were not statistically different (p > 0.05). No effect of IVF technique was seen on the success rate (6%) in each group.
Authors:
S Deleuze; C S Dubois; M Caillaud; B Bruneau; G Goudet; G Duchamp
Publication Detail:
Type:  Journal Article     Date:  2008-10-28
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  45     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-08     Completed Date:  2010-04-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Affiliation:
Facult? de M?decine V?t?rinaire, D?partement des Sciences Cliniques-Clinique Equine, Universit? de Li?ge, Li?ge, Belgium. s.deleuze@ulg.ac.be
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / drug effects,  physiology
Cells, Cultured
Cysteamine / pharmacology*
Cytoplasm / drug effects,  physiology
Embryo, Mammalian / physiology
Fallopian Tubes
Female
Fertilization / drug effects*
Fertilization in Vitro / drug effects,  veterinary*
Horses*
Insemination, Artificial / veterinary
Male
Oocytes / drug effects*,  transplantation,  ultrastructure
Spermatozoa / physiology
Tissue and Organ Harvesting / methods,  veterinary
Chemical
Reg. No./Substance:
60-23-1/Cysteamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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