Document Detail


The influence of collagen network integrity on the accumulation of gadolinium-based mr contrast agents in articular cartilage.
MedLine Citation:
PMID:  21058237     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
PURPOSE: Delayed gadolinium-enhanced MR imaging of cartilage is used to quantify the proteoglycan loss in early osteoarthritis. It is assumed that T1 after Gd-DTPA administration in the near equilibrium state reflects selective proteoglycan loss from cartilage. To investigate the influence of the collagen network integrity on contrast accumulation, the relaxation rates ΔR(1) and ΔR(2) were compared after Gd-DTPA administration in a well established model of osteoarthritis.
MATERIALS AND METHODS: Collagen or proteoglycan depletion was induced by the proteolytic enzymes papain and collagenase in healthy bovine patellar cartilage. Using a dedicated MRI sequence, T(1) and T(2) maps were simultaneously acquired before and 11 h after Gd-DTPA administration. Depth-dependent profiles of ΔR(1) and ΔR(2) were calculated in healthy, proteoglycan and collagen-depleted articular cartilage and the mean values of different cartilage layers were compared using the Mann-Whitney-U test.
RESULTS: In superficial layers (1 mm) there was no significant difference (p > 0.05) in either ΔR(1) or ΔR(2) between proteoglycan-depleted (16.6 ± 1.2 s(-1), 15.9 ± 1.0 s(-1)) and collagen-depleted articular cartilage (15.3 ± 0.9 s(-1), 15.5 ± 0.9 s(-1)). In deep layers (3 mm) both parameters were significantly higher (p = 0.005, 0.03) in proteoglycan-depleted articular cartilage (12.3 ± 1.1 s(-1), 9.8 ± 0.8 s(-1)) than in collagen-depleted articular cartilage (9.1 ± 1.1 s(-1), 8.7 ± 0.7 s(-1)).
CONCLUSION: Both proteoglycan loss and alterations in the collagen network influence the accumulation of Gd-DTPA in articular cartilage with significant differences between superficial and deep cartilage layers.
Authors:
E Wiener; M Settles; G Weirich; C Schmidt; G Diederichs
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Publication Detail:
Type:  Journal Article     Date:  2010-11-05
Journal Detail:
Title:  RöFo : Fortschritte auf dem Gebiete der Röntgenstrahlen und der Nuklearmedizin     Volume:  183     ISSN:  1438-9010     ISO Abbreviation:  Rofo     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7507497     Medline TA:  Rofo     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  226-32     Citation Subset:  IM    
Copyright Information:
© Georg Thieme Verlag KG Stuttgart · New York.
Affiliation:
Institut für Radiologie, Charité Universitätsmedizin Berlin, Berlin. edzard.wiener@charite.de
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