| Influence of Cholesterol and β-Sitosterol on the Structure of EYPC Bilayers. | |
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MedLine Citation:
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PMID: 21814861 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The influence of cholesterol and β-sitosterol on egg yolk phosphatidylcholine (EYPC) bilayers is compared. Different interactions of these sterols with EYPC bilayers were observed using X-ray diffraction. Cholesterol was miscible with EYPC in the studied concentration range (0-50 mol%), but crystallization of β-sitosterol in EYPC bilayers was observed at X ≥ 41 mol% as detected by X-ray diffraction. Moreover, the repeat distance (d) of the lamellar phase was similar upon addition of the two sterols up to mole fraction 17%, while for X ≥ 17 mol% it became higher in the presence of β-sitosterol compared to cholesterol. SANS data on suspensions of unilamellar vesicles showed that both cholesterol and β-sitosterol similarly increase the EYPC bilayer thickness. Cholesterol in amounts above 33 mol% decreased the interlamellar water layer thickness, probably due to "stiffening" of the bilayer. This effect was not manifested by β-sitosterol, in particular due to the lower solubility of β-sitosterol in EYPC bilayers. Applying the formalism of partial molecular areas, it is shown that the condensing effect of both sterols on the EYPC area at the lipid-water interface is small, if any. The parameters of ESR spectra of spin labels localized in different regions of the EYPC bilayer did not reveal any differences between the effects of cholesterol and β-sitosterol in the range of full miscibility. |
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Authors:
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Jana Gallová; Daniela Uhríková; Norbert Kučerka; Miroslava Svorková; Sergio S Funari; Tatiana N Murugova; László Almásy; Milan Mazúr; Pavol Balgavý |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-8-4 |
Journal Detail:
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Title: The Journal of membrane biology Volume: - ISSN: 1432-1424 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-8-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0211301 Medline TA: J Membr Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Physical Chemistry of Drugs, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32, Bratislava, Slovakia, gallova@fpharm.uniba.sk. |
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