Document Detail


Influence of the CNS environment on chromaffin cell survival and catecholamine secretion patterns.
MedLine Citation:
PMID:  8051558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromaffin cells implanted into the CNS have been used as a potential source of sustained catecholamine delivery, although their survival and continued catecholamine secretion are controversial. In addition, chromaffin cells exhibit a high degree of neurochemical plasticity in response to environmental factors. The present aims were to determine whether the CNS provides a supportive environment for sustained catecholamine production in transplanted chromaffin cells and to assess whether this novel environment alters patterns of catecholamine secretion. Catecholamine release from bovine chromaffin cells implanted into the rat midbrain was determined in brain slices. In addition, alterations in catecholamine secretion patterns, particularly adrenaline/noradrenaline ratios, were compared in vitro versus in transplants. Results indicated that brain slices containing chromaffin cell implants released high basal and nicotine-stimulated levels of adrenaline and noradrenaline. It is surprising that although adrenaline/noradrenaline ratios steadily declined in culture, this did not occur when cells were transplanted to the CNS in the early postharvesting phases. However, if cells were transplanted following longer periods in culture, adrenaline/noradrenaline ratios remained low. Together, these results suggest that the CNS can provide a supportive environment for chromaffin cell survival and that the pattern of catecholamine secretion can be optimized by prior in vitro manipulation.
Authors:
J Sagen; J D Ortega
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  63     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1994-09-08     Completed Date:  1994-09-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1159-62     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Cell Biology, University of Illinois at Chicago 60612.
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MeSH Terms
Descriptor/Qualifier:
Adrenal Glands / cytology,  transplantation
Animals
Catecholamines / secretion*
Cattle
Cell Survival
Cells, Cultured
Chromaffin System / cytology,  physiology*
Epinephrine / secretion
Mesencephalon / physiology*
Norepinephrine / secretion
Time Factors
Grant Support
ID/Acronym/Agency:
NS25054/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Catecholamines; 51-41-2/Norepinephrine; 51-43-4/Epinephrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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