Document Detail

Influence of 6-diazo-5-oxo-L-norleucine (DON), a glutamine analogue, on cartilaginous differentiation in mouse limb buds in vitro.
MedLine Citation:
PMID:  1183764     Owner:  NLM     Status:  MEDLINE    
The antibiotic, 6-diazo-5-oxo-L-norleucine (DON), an analogue of L-glutamine, causes limb malformations in several species, including mice. This report shows that DON also interferes with differentiation of cartilaginous rudiments of mouse limb buds grown as organ cultures for 3 to 8 days in medium containing the teratogen. DON (5 mug/ml) inhibits growth of the explants and interferes with production of normal matrix by chondrocytes. The cartilage of DON-treated cultures exhibits a striking lack of matrix, compared with that of control explants which contains abundant metachromatic matrix. Differentiation of osteoblasts, and secretion of osteoid around the scapula and humerus are enhanced by DON. The direct effects of DON on growth and chondrogenesis, which can be prevented by addition of L-glutamine (1 mg/ml) to the medium, can be attributed to the known interference of DON in L-glutamine-dependent steps in metabolism. The possible relationships between these effects of DON in vitro and the malformations produced in vivo, are discussed.
M B Aydelotte; D M Kochhar
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  4     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  1975 Oct 
Date Detail:
Created Date:  1976-01-23     Completed Date:  1976-01-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  73-80     Citation Subset:  IM    
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MeSH Terms
Azo Compounds / pharmacology*
Cartilage / drug effects,  embryology*
Cell Differentiation / drug effects
Diazooxonorleucine / pharmacology*
Dose-Response Relationship, Drug
Embryo, Mammalian / drug effects
Extremities / drug effects,  embryology*
Glutamine / pharmacology
Mice, Inbred C57BL
Mice, Inbred DBA
Morphogenesis / drug effects*
Organ Culture Techniques
Reg. No./Substance:
0/Azo Compounds; 56-85-9/Glutamine; 764-17-0/Diazooxonorleucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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