Document Detail

Influence of 17beta-estradiol and progesterone on GABAergic gene expression in the arcuate nucleus, amygdala and hippocampus of the rhesus macaque.
MedLine Citation:
PMID:  19833106     Owner:  NLM     Status:  MEDLINE    
Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the brain, and the responsiveness of neurons to GABA can be modulated by sex steroids. To better understand how ovarian steroids influence the GABAergic system in the primate brain, we evaluated the expression of genes encoding GABA receptor subunits, glutamic acid decarboxylase (GAD) and a GABA transporter in the brains of female rhesus macaques. Ovariectomized adults were subjected to a hormone replacement paradigm involving either 17beta-estradiol (E), or E plus progesterone (E+P). Untreated animals served as controls. Using GeneChip microarray analysis and real-time RT-PCR (qPCR), we examined gene expression differences within and between the amygdala (AMD), hippocampus (HPC) and arcuate nuclei of the medial basal hypothalamus (MBH). The results from PCR corresponded with results from representative GeneChip probesets, and showed similar effects of sex steroids on GABA receptor subunit gene expression in the AMD and HPC, and a more pronounced expression than in the MBH. Exposure to E+P attenuated GAD1, GAD2 and SLC32A1 gene expression in the AMD and HPC, but not in the MBH. GABA receptor subunit gene expression was generally higher in the AMD and HPC than in the MBH, with the exception of receptor subunits epsilon and gamma 2. Taken together, the data demonstrate differential regulation of GABA receptor subunits and GABAergic system components in the MBH compared to the AMD and HPC of rhesus macaques. Elevated epsilon and reduced delta subunit expression in the MBH supports the hypothesis that the hypothalamic GABAergic system is resistant to the modulatory effects of sex steroids.
Nigel C Noriega; Dominique H Eghlidi; Vasilios T Garyfallou; Steven G Kohama; Sharon G Kryger; Henryk F Urbanski
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-10-13
Journal Detail:
Title:  Brain research     Volume:  1307     ISSN:  1872-6240     ISO Abbreviation:  Brain Res.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-01     Completed Date:  2010-03-08     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  28-42     Citation Subset:  IM    
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MeSH Terms
Amygdala / drug effects
Arcuate Nucleus / drug effects
Brain / anatomy & histology*,  drug effects*
Estradiol / pharmacology*
Estrogens / pharmacology*
Gene Expression / drug effects*
Gene Expression Profiling / methods
Hippocampus / drug effects
Macaca mulatta
Oligonucleotide Array Sequence Analysis / methods
Ovariectomy / methods
Progesterone / pharmacology*
Progestins / pharmacology*
Receptors, GABA / genetics,  metabolism
Vesicular Inhibitory Amino Acid Transport Proteins / genetics,  metabolism
gamma-Aminobutyric Acid / genetics,  metabolism*
Grant Support
AG026472/AG/NIA NIH HHS; AG029612/AG/NIA NIH HHS; HD029186/HD/NICHD NIH HHS; P51 RR000163/RR/NCRR NIH HHS; P51 RR000163-420018/RR/NCRR NIH HHS; P51 RR000163-430018/RR/NCRR NIH HHS; P51 RR000163-508714/RR/NCRR NIH HHS; P51 RR000163-508756/RR/NCRR NIH HHS; R01 AG029612/AG/NIA NIH HHS; R01 AG029612-02S1/AG/NIA NIH HHS; R01 AG029612-03/AG/NIA NIH HHS; R01 HD029186/HD/NICHD NIH HHS; R01 HD029186-08/HD/NICHD NIH HHS; R21 AG026472/AG/NIA NIH HHS; R21 AG026472-02/AG/NIA NIH HHS; RR000163/RR/NCRR NIH HHS
Reg. No./Substance:
0/Estrogens; 0/Progestins; 0/Receptors, GABA; 0/Vesicular Inhibitory Amino Acid Transport Proteins; 4G7DS2Q64Y/Progesterone; 4TI98Z838E/Estradiol; 56-12-2/gamma-Aminobutyric Acid

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