Document Detail


Inflammatory cytokines and cell death in BEAS-2B lung cells treated with soil dust, lipopolysaccharide, and surface-modified particles.
MedLine Citation:
PMID:  15310859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultured human lung epithelial cells (BEAS-2B) were treated in vitro with PM(2.5)-enriched particles of soil-derived mineral dust from nine sites in the western United States. The particle samples simulate windblown dust and vehicle-generated emissions from unpaved roads. Five of the sites yielded relatively benign dust. Particles from three sites caused IL-6 release when cells were treated for 24 h at doses from 20 to 80 microg/cm(2), and particles from one site were highly cytotoxic. The particle components or characteristics that caused the IL-6 release were stable at temperatures below 150 degrees C, but were inactivated by treatment at 300-550 degrees C. The active factors were also associated predominantly with the insoluble fraction, and were partially attenuated by leaching with aqueous and organic solvents. The IL-6 release caused by the particles was much greater than the cytokine response to either lipopolysaccharide (LPS) or to surrogate particles of titanium dioxide mixed with LPS, suggesting that endotoxin was not a major factor in the inflammatory response. The release of IL-8 in response to particle treatment was qualitatively similar to the IL-6 response, but release of TNF-alpha was not detected at the 24-h time point. The combined results support the hypothesis that some ambient dusts from geological sources can cause cell death and cytokine release in a lung cell line that is widely used as an in vitro model to study mechanisms of environmental respiratory injury.
Authors:
John M Veranth; Christopher A Reilly; Martha M Veranth; Tyler A Moss; Charles R Langelier; Diane L Lanza; Garold S Yost
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2004-08-13
Journal Detail:
Title:  Toxicological sciences : an official journal of the Society of Toxicology     Volume:  82     ISSN:  1096-6080     ISO Abbreviation:  Toxicol. Sci.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-10-19     Completed Date:  2005-02-22     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  9805461     Medline TA:  Toxicol Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  88-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Air Pollutants
Bronchi / cytology,  drug effects*,  secretion
Cell Line
Cell Survival / drug effects
Dose-Response Relationship, Drug
Drug Combinations
Dust* / analysis
Epithelial Cells / cytology,  drug effects,  secretion
Humans
Interleukin-6 / secretion*
Interleukin-8 / secretion*
Lipopolysaccharides / pharmacology*
Particle Size
Pseudomonas aeruginosa / immunology
Soil* / analysis
Surface Properties
Titanium / pharmacology
Tumor Necrosis Factor-alpha / secretion
Grant Support
ID/Acronym/Agency:
HL 069813/HL/NHLBI NIH HHS; HL 13645/HL/NHLBI NIH HHS; K25 ES 011281/ES/NIEHS NIH HHS; K25 ES011281-02/ES/NIEHS NIH HHS; R01 HL013645/HL/NHLBI NIH HHS; R01 HL013645-30/HL/NHLBI NIH HHS; R01 HL069813/HL/NHLBI NIH HHS; R01 HL069813-01A1/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Air Pollutants; 0/Drug Combinations; 0/Dust; 0/Interleukin-6; 0/Interleukin-8; 0/Lipopolysaccharides; 0/Soil; 0/Tumor Necrosis Factor-alpha; 15FIX9V2JP/titanium dioxide; D1JT611TNE/Titanium
Comments/Corrections

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