| Inflammatory cytokine release of astrocytes in vitro is reduced by all-trans retinoic acid. | |
| | |
MedLine Citation:
|
PMID: 20826012 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In the central nervous system inflammation is mediated by microglia and astrocytes. To investigate its regulation, murine astrocyte cultures were treated with bacterial lipopolysaccharides (LPS) and analyzed with Affymetrix gene array, qRT-PCR and ELISA. Cells responded to LPS with a strong upregulation of pro-inflammatory cytokines and chemokines. Treatment with the transcriptional activator retinoic acid (RA) suppressed mRNA expression and protein release of several important cytokines (IL-1β 4%, IL-6 21%, TNFα 30%, IL-12p40 42%, and IL-12p35/p40 27%; p<0.01). The data are consistent with the hypothesis that all-trans RA takes part in endogenous anti-inflammatory feedback loops in the CNS. |
| | |
Authors:
|
Sabien van Neerven; Andrei Nemes; Philipp Imholz; Tommy Regen; Bernd Denecke; Sonja Johann; Cordian Beyer; Uwe-Karsten Hanisch; Jörg Mey |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-09 |
Journal Detail:
|
Title: Journal of neuroimmunology Volume: 229 ISSN: 1872-8421 ISO Abbreviation: J. Neuroimmunol. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-24 Completed Date: 2010-12-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8109498 Medline TA: J Neuroimmunol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 169-79 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
Institut für Biologie II, RWTH Aachen University, Aachen, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Analysis of Variance Animals Animals, Newborn Annexin A2 / metabolism Anti-Inflammatory Agents / pharmacology Antineoplastic Agents / pharmacology* Astrocytes / drug effects* Cells, Cultured Cerebral Cortex / cytology Clodronic Acid / pharmacology Cytokines / genetics, metabolism* Dexamethasone / pharmacology Drug Interactions Enzyme-Linked Immunosorbent Assay / methods Gene Expression Profiling / methods Gene Expression Regulation / drug effects Glial Fibrillary Acidic Protein / metabolism L-Lactate Dehydrogenase / metabolism Lipopolysaccharides / pharmacology* Mice Mice, Inbred BALB C Oligonucleotide Array Sequence Analysis / methods RNA, Messenger / metabolism Retinoid X Receptors / metabolism Tretinoin / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Annexin A2; 0/Anti-Inflammatory Agents; 0/Antineoplastic Agents; 0/Cytokines; 0/Glial Fibrillary Acidic Protein; 0/Lipopolysaccharides; 0/RNA, Messenger; 0/Retinoid X Receptors; 10596-23-3/Clodronic Acid; 302-79-4/Tretinoin; 50-02-2/Dexamethasone; EC 1.1.1.27/L-Lactate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: T cells that trigger acute experimental autoimmune encephalomyelitis also mediate subsequent disease...
Next Document: Intrauterine infection/inflammation and perinatal brain damage: role of glial cells and Toll-like re...