Document Detail


Inflammation induced by innate immunity in the central nervous system leads to primary astrocyte dysfunction followed by demyelination.
MedLine Citation:
PMID:  20532539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Primary loss and dysfunction of astrocytes may trigger demyelination, as seen in neuromyelitis optica, an inflammatory disease of the central nervous system. In most patients affected by this disease, injury to astrocytes is initiated by the action of autoantibodies targeting aquaporin 4 (AQP-4), a water channel on astrocytes. We show here that damage of astrocytes and subsequent demyelination can also occur in the absence of autoantibody-mediated mechanisms. Following injection of lipopolysaccharide into the white matter initial microglia activation is followed by a functional disturbance of astrocytes, mainly reflected by retraction of astrocytic foot processes at the glia limitans and loss of AQP-4 and connexins, which are involved in the formation of gap junctions between astrocytes and oligodendrocytes. Demyelination and oligodendrocyte degeneration in this model follows astrocyte pathology. Similar structural abnormalities were also seen in a subset of active lesions in multiple sclerosis. Our studies suggest that astrocyte injury may be an important early step in the cascade of lesion formation in brain inflammation.
Authors:
Rakhi Sharma; Marie-Therese Fischer; Jan Bauer; Paul A Felts; Kenneth J Smith; Tatsuro Misu; Kazuo Fujihara; Monika Bradl; Hans Lassmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-08
Journal Detail:
Title:  Acta neuropathologica     Volume:  120     ISSN:  1432-0533     ISO Abbreviation:  Acta Neuropathol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-06-28     Completed Date:  2010-09-28     Revised Date:  2011-04-06    
Medline Journal Info:
Nlm Unique ID:  0412041     Medline TA:  Acta Neuropathol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  223-36     Citation Subset:  IM    
Affiliation:
Centre for Brain Research, Medical University of Vienna, Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aquaporin 4 / metabolism
Astrocytes / immunology*,  pathology
Cells, Cultured
Central Nervous System / pathology*
Coculture Techniques / methods
Disease Models, Animal
Encephalomyelitis, Autoimmune, Experimental* / etiology,  immunology,  pathology
Gene Expression Regulation / drug effects,  immunology
Humans
Immunity, Innate*
Membrane Proteins / metabolism
Microscopy, Confocal / methods
Multiple Sclerosis / immunology,  pathology*
Nerve Tissue Proteins / metabolism
Neuroglia / drug effects
Polysaccharides / pharmacology
Rats
Time Factors
Grant Support
ID/Acronym/Agency:
P 19854-B02//Austrian Science Fund FWF; P 21581-B09//Austrian Science Fund FWF
Chemical
Reg. No./Substance:
0/AQP4 protein, human; 0/Aquaporin 4; 0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/Polysaccharides
Comments/Corrections

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