| Inflammation in fetal sheep from intra-amniotic injection of Ureaplasma parvum. | |
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MedLine Citation:
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PMID: 20935228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bronchopulmonary dysplasia is associated with chorioamnionitis and fetal lung inflammation. Ureaplasma species are the bacteria most frequently isolated from chorioamnionitis. Very chronic ureaplasma colonization of amniotic fluid causes low-grade lung inflammation and functional lung maturation in fetal sheep. Less is known about shorter exposures of the fetal lung. Therefore, we hypothesized that ureaplasmas would cause an acute inflammatory response that would alter lung development. Singleton ovine fetuses received intra-amniotic Ureaplasma parvum serovar 3 or control media at 110, 117, or 121 days and were delivered at 124 days gestational age (term = 150 days). Inflammation was assessed by 1) cell counts in bronchoalveolar lavage fluid (BALF), and 2) cytokine mRNA measurements, immunohistochemistry, and flow cytometry for inflammatory cells and elastin and α-smooth muscle actin (α-SMA) staining in lung tissue. Neutrophils were increased in BALF 3 days after exposure to ureaplasmas (P = 0.01). Myeloperoxidase-positive cells increased after 3 days (P = 0.03), and major histocompatibility complex (MHC) class II-positive cells increased after 14 days of ureaplasma exposure (P = 0.001). PU.1 (macrophage marker)- or CD3 (T lymphocyte marker)-positive cells were not induced by ureaplasmas. CD3-positive cells in the posterior mediastinal lymph node increased in ureaplasma-exposed animals at 3, 7, and 14 days (P = 0.002). Focal elastin depositions decreased in alveolar septa at 14 days (P = 0.002), whereas α-SMA increased in arteries and bronchioli. U. parvum induced a mild acute inflammatory response and changed elastin and α-SMA deposition in the lung, which may affect lung structure and subsequent development. |
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Authors:
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Jennifer J P Collins; Suhas G Kallapur; Christine L Knox; Ilias Nitsos; Graeme R Polglase; J Jane Pillow; Elke Kuypers; John P Newnham; Alan H Jobe; Boris W Kramer |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-08 |
Journal Detail:
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Title: American journal of physiology. Lung cellular and molecular physiology Volume: 299 ISSN: 1522-1504 ISO Abbreviation: Am. J. Physiol. Lung Cell Mol. Physiol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-06 Completed Date: 2011-01-13 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 100901229 Medline TA: Am J Physiol Lung Cell Mol Physiol Country: United States |
Other Details:
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Languages: eng Pagination: L852-60 Citation Subset: IM |
Affiliation:
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Dept. of Pediatrics, Maastricht Univ. Medical Center, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bronchoalveolar Lavage Fluid / cytology Cytokines / genetics, metabolism Female Fetus* / immunology, microbiology, physiopathology Gestational Age Humans Male Pneumonia* / immunology, microbiology, pathology Pregnancy Sheep* Ureaplasma / pathogenicity* Ureaplasma Infections / immunology*, metabolism, pathology |
| Grant Support | |
ID/Acronym/Agency:
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HD-57869/HD/NICHD NIH HHS; R01 HD057869-03/HD/NICHD NIH HHS; R01-HL-97064/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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