Document Detail


Inflammation is associated with a decrease of lipogenic factors in omental fat in women.
MedLine Citation:
PMID:  18448614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Obesity is characterized by systemic low-grade inflammation in which adipose tissue, especially the omental depot, is thought to play a key role. We have previously shown that inflammation impairs 3T3-L1 preadipocyte cell line differentiation. To explore whether this interaction also takes place in vivo, the expression of several genes related to inflammation and adipocyte differentiation was assessed in human samples. Paired adipose tissue biopsies (from omental and subcutaneous depots) were obtained from 24 women: 6 lean normoglycemic and 18 obese volunteers with different glycemic states (normoglycemic, glucose-intolerant, or type 2 diabetic). The expression levels of CD14, IL-18, leptin, adiponectin, sterol regulatory element binding transcription factor 1 (SREBP1), peroxisome proliferator-activated receptor gamma (PPARgamma), pre-B-cell colony enhancing factor 1 (PBEF1) (or visfatin), glycerol-3-phosphate dehydrogenase 1 (soluble) (GPD1), lipoprotein lipase (LPL), fatty acid binding protein 4, adipocyte (FABP4), and hypoxia-inducible factor 1alpha were determined by quantitative real-time PCR. CD14 and IL-18 were overexpressed in omental adipose tissue compared with the subcutaneous depot, irrespective of the subject's obesity or diabetes status. A significant decrease of LPL, GPD1, and leptin expression was observed in omental tissue, and an inverse correlation between expression of CD14 and IL-18 and that of PPARgamma, LPL, and FABP4 was observed. The underexpression of omental lipogenic markers was more accentuated in the presence of glucose intolerance. Furthermore, adiponectin and SREBP1 expression was also significantly decreased in omental tissue of type 2 diabetic patients. PBEF1 and HIF1alpha expression remained comparable in all samples. Therefore, in humans, inflammation is increased in the omental depot, as evidenced by CD14 and IL-18 expression. In this localization, the inflammatory state is associated with a decreased expression of lipogenic markers, which is more pronounced in diabetic subjects.
Authors:
Odile Poulain-Godefroy; Cécile Lecoeur; François Pattou; Gema Frühbeck; Philippe Froguel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-30
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  295     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-10     Completed Date:  2008-09-03     Revised Date:  2008-12-04    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R1-7     Citation Subset:  IM    
Affiliation:
Centre National de la Recherche Scientifique 8090-Institute of Biology, Pasteur Institute, Lille, France. odile.poulain@good.ibl.fr
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MeSH Terms
Descriptor/Qualifier:
Adiponectin / genetics,  metabolism
Adipose Tissue / physiology*
Adult
Antigens, CD14 / genetics,  metabolism
Body Composition
Cytokines / genetics,  metabolism
Diabetes Mellitus, Type 2 / complications,  metabolism
Fatty Acid-Binding Proteins / genetics,  metabolism
Female
Gene Expression Regulation / physiology
Glucose Intolerance / complications,  metabolism
Glycerolphosphate Dehydrogenase / genetics,  metabolism
Humans
Hypoxia-Inducible Factor 1, alpha Subunit / genetics,  metabolism
Inflammation / metabolism*
Interleukin-18 / genetics,  metabolism
Leptin / genetics,  metabolism
Lipogenesis / physiology*
Lipoprotein Lipase / genetics,  metabolism
Middle Aged
Nicotinamide Phosphoribosyltransferase / genetics,  metabolism
Obesity / complications,  metabolism
Omentum / physiology*
PPAR gamma / genetics,  metabolism
RNA, Messenger / genetics,  metabolism
Sterol Regulatory Element Binding Protein 1 / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Adiponectin; 0/Antigens, CD14; 0/Cytokines; 0/FABP4 protein, human; 0/Fatty Acid-Binding Proteins; 0/HIF1A protein, human; 0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/Interleukin-18; 0/Leptin; 0/PPAR gamma; 0/RNA, Messenger; 0/SREBF1 protein, human; 0/Sterol Regulatory Element Binding Protein 1; EC 1.1.-/GPD1-L protein, human; EC 1.1.-/Glycerolphosphate Dehydrogenase; EC 2.4.2.12/Nicotinamide Phosphoribosyltransferase; EC 2.4.2.12/nicotinamide phosphoribosyltransferase, human; EC 3.1.1.34/Lipoprotein Lipase
Comments/Corrections
Comment In:
Am J Physiol Regul Integr Comp Physiol. 2008 Oct;295(4):R1097; author reply R1098   [PMID:  18832093 ]

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