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Inflammasome activation restricts Legionella pneumophila replication in primary microglial cells through flagellin detection.
MedLine Citation:
PMID:  23355222     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Microglial cells constitute the first line of defense of the central nervous system (CNS) against microbial invasion. Pathogens are detected thanks to an array of innate immune receptors termed pattern recognition receptors (PRRs). PRRs have been thoroughly characterized in bone marrow-derived macrophages, but the PRRs repertoire and functionality in microglial cells remain largely unknown. Microglial cells express various Toll-like Receptors and the Nod1/2 receptors. Recently, a novel innate immune signalling pathway, the inflammasome pathway has been uncovered. Inflammasome activation leads to caspase-1 activation, release of the proinflammatory cytokines, IL-1β and IL-18 and cell death in a process termed pyroptosis. One inflammasome receptor, NLRP3, has been characterized in microglial cells and associated with response to infections and in the initiation of neuro-degeneration in an Alzheimer's disease model. Legionella pneumophila (L.pneumophila) is a flagellated bacterium replicating within macrophages. In bone marrow-derived macrophages, L. pneumophila is detected in a flagellin-dependent manner by the Naip5-NLRC4 (Ipaf) inflammasome pathway. In this study, we decided to use L. pneumophila to investigate the presence and the functionality of this inflammasome in primary murine microglial cells. We show that microglial cells detect L. pneumophila infection in a flagellin-dependent manner leading to caspase-1-mediated bacterial growth restriction, infected cell death and secretion of the proinflammatory cytokines IL-1β and IL18. Overall, our data demonstrate that microglial cells have a functional Naip5-NLRC4 inflammasome likely to be important to monitor and clear CNS infections by flagellated bacteria. © 2013 Wiley Periodicals, Inc. © 2013 Wiley Periodicals, Inc.
Authors:
Yvan Jamilloux; Roberto Pierini; Mathieu Querenet; Carole Juruj; Anne-Laure Fauchais; Marie-Odile Jauberteau; Sophie Jarraud; Gérard Lina; Jérôme Etienne; Craig R Roy; Thomas Henry; Nathalie Davoust-Nataf; Florence Ader
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-28
Journal Detail:
Title:  Glia     Volume:  -     ISSN:  1098-1136     ISO Abbreviation:  Glia     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
Affiliation:
Bacterial Pathogenesis and Innate Immunity laboratory, Inserm U851 "Immunity, Infection and Vaccination," Claude Bernard Lyon I University, Lyon, France; EA 3842, "Homéostasie Cellulaire et Pathologies," Limoges University Hospital, 87025 Limoges, France.
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