Document Detail

Infiltration of the kidney by alpha beta and gamma delta T cells: effect on progression in IgA nephropathy.
MedLine Citation:
PMID:  7731144     Owner:  NLM     Status:  MEDLINE    
We have studied renal biopsies from three groups of patients to determine if alpha beta T cells or gamma delta T cells are present, and whether their presence is correlated with disease progression in IgA nephropathy (IgAN). Group one comprised thin basement membrane disease biopsies (non-immunological control, N = 7); group two were patients with IgAN and stable renal function one year following biopsy (stable, N = 7); and group three were IgAN patients with rapidly declining renal function after one year (progressive, N = 7). Immunohistochemical staining using monoclonal antibodies (CD3, TcR beta, TcR delta) and molecular studies utilizing polymerase chain reaction amplification of cDNA transcribed from biopsy RNA, with primers specific for either the alpha beta TcR or gamma delta TcR, were undertaken. On immunohistochemistry a significant increase in CD3 + cells in progressive biopsies was seen (vs. control P = 0.002, vs. stable P = 0.002). The progressive biopsies infiltrate consisted of both alpha beta TcR (vs. control P = 0.001, vs. stable P = 0.003) and gamma delta TcR cells (vs. control P = 0.01). The RNA study demonstrated an increase in TcR C alpha transcription in the progressive (vs. control P = 0.003) biopsies. Increased TcR C delta transcription was seen in the progressive group (vs. control P = 0.01, vs. stable P = 0.02). We confirm that the presence of lymphocytes in IgAN biopsies predicts progressive disease. While alpha beta T cells are found in both stable and progressive disease, the presence of gamma delta T cells is only associated with progressive IgAN.
M C Falk; G Ng; G Y Zhang; G C Fanning; L P Roy; K M Bannister; A C Thomas; A R Clarkson; A J Woodroffe; J F Knight
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Kidney international     Volume:  47     ISSN:  0085-2538     ISO Abbreviation:  Kidney Int.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-06-01     Completed Date:  1995-06-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0323470     Medline TA:  Kidney Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  177-85     Citation Subset:  IM    
Renal Research Laboratories, Children's Hospital, Camperdown, New South Wales, Australia.
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MeSH Terms
Antibodies, Monoclonal
Base Sequence
Cell Movement
DNA Primers / chemistry
DNA, Complementary / analysis
Disease Progression
Glomerulonephritis, IGA / immunology*,  pathology
Immunoenzyme Techniques
Kidney / immunology*,  pathology
Middle Aged
Molecular Sequence Data
Polymerase Chain Reaction
Receptors, Antigen, T-Cell, alpha-beta / chemistry,  immunology*
Receptors, Antigen, T-Cell, gamma-delta / chemistry,  immunology*
T-Lymphocytes / immunology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/DNA Primers; 0/DNA, Complementary; 0/Receptors, Antigen, T-Cell, alpha-beta; 0/Receptors, Antigen, T-Cell, gamma-delta

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