Document Detail


Inferring the palaeoenvironment of ancient bacteria on the basis of resurrected proteins.
MedLine Citation:
PMID:  13679914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Features of the physical environment surrounding an ancestral organism can be inferred by reconstructing sequences of ancient proteins made by those organisms, resurrecting these proteins in the laboratory, and measuring their properties. Here, we resurrect candidate sequences for elongation factors of the Tu family (EF-Tu) found at ancient nodes in the bacterial evolutionary tree, and measure their activities as a function of temperature. The ancient EF-Tu proteins have temperature optima of 55-65 degrees C. This value seems to be robust with respect to uncertainties in the ancestral reconstruction. This suggests that the ancient bacteria that hosted these particular genes were thermophiles, and neither hyperthermophiles nor mesophiles. This conclusion can be compared and contrasted with inferences drawn from an analysis of the lengths of branches in trees joining proteins from contemporary bacteria, the distribution of thermophily in derived bacterial lineages, the inferred G + C content of ancient ribosomal RNA, and the geological record combined with assumptions concerning molecular clocks. The study illustrates the use of experimental palaeobiochemistry and assumptions about deep phylogenetic relationships between bacteria to explore the character of ancient life.
Authors:
Eric A Gaucher; J Michael Thomson; Michelle F Burgan; Steven A Benner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nature     Volume:  425     ISSN:  1476-4687     ISO Abbreviation:  Nature     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-18     Completed Date:  2003-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  England    
Other Details:
Languages:  eng     Pagination:  285-8     Citation Subset:  IM    
Affiliation:
NASA Astrobiology Institute, Gainesville, Florida 32611-7200, USA. gaucher@ufl.edu
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY305395;  AY305396;  AY305397
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MeSH Terms
Descriptor/Qualifier:
Bacteria / classification,  metabolism*
Bacterial Proteins / metabolism*
Base Composition
Environment*
Enzyme Stability
Guanosine Diphosphate / metabolism
Molecular Sequence Data
Peptide Elongation Factor Tu / metabolism*
Phylogeny
Protein Binding
RNA, Ribosomal / genetics
Temperature
Time Factors
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/RNA, Ribosomal; 146-91-8/Guanosine Diphosphate; EC 3.6.1.-/Peptide Elongation Factor Tu

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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