Document Detail


Infectivity of penicillin-induced Chlamydia psittaci forms for cell cultures from the chorioallantoic membrane of chick embryos.
MedLine Citation:
PMID:  6999778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
L-cells were infected at high multiplicity with meningopneumonitis organsims (MP) and incubated in medium with penicillin (200 units/ml) and without the drug. At intervals up to 36 hours cells were removed and L-cell associated chlamydial infectivity was determined for cell cultures from the chorioallantoic membrane (CAM) of chick embryos which were treated before inoculation with Diethylaminoethyl (DEAE)-Dextran. In control L-cells, the infectivity decreased at 8 hours and reached it lowest level 16 hours after infection. In penicillin-treated L-cells in contrast, infectivity remained at low levels from 2 and up to 36 hours after infection. At 16 hours after infection about 90% of both penicillin-treated and nontreated L-cells contained single or multiple Giemsa-stained intracytoplasmic inclusions which in both series contained almost exclusively reticulate bodies (RB) by electron microscopy. RB from 16 hour infected penicillin-treated and non-treated L-cells were partially freed from cell debris and their infectivity (ID50) for CAM-culture cells (TC) was assayed 3 times. Penicillin-induced RB had TCID50 of 10(4.0)/ml compared to control RB of 10(4.5)/ml. The TCID50 of the original MP strain for CAM-culture cells was 10(8.0)/ml. Penicillin-induced RB and control RB from 16 hour L-cell samples were frozen and thawed 3 times and titrated in CAM cells; the TCID50 of both series dropped to 10(1.0)/ml while the TCID50 of the original MP inoculum after freezing and thawing 3 times was 10(7.0)/ml.
Authors:
N Kordová; J C Wilt; M R Neuman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zentralblatt für Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie     Volume:  246     ISSN:  0172-5599     ISO Abbreviation:  Zentralbl Bakteriol A     Publication Date:  1980 Feb 
Date Detail:
Created Date:  1980-12-18     Completed Date:  1980-12-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8005748     Medline TA:  Zentralbl Bakteriol A     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  119-27     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Chick Embryo
Chlamydophila psittaci / drug effects,  pathogenicity*
Chorion
DEAE-Dextran
Fluorescent Antibody Technique
L Cells (Cell Line)
Penicillins / pharmacology
Chemical
Reg. No./Substance:
0/Penicillins; 9015-73-0/DEAE-Dextran

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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