| Infectivity of Chlorella species for the ciliate Paramecium bursaria is not based on sugar residues of their cell wall components, but on their ability to localize beneath the host cell membrane after escaping from the host digestive vacuole in the early infection process. | |
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MedLine Citation:
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PMID: 17602279 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Paramecium bursaria cells harbor several hundred symbiotic algae in their cytoplasm. Algae-free cells can be reinfected with algae isolated from algae-bearing cells or cultivated Chlorella species through the digestive vacuoles. To determine the relationship between the infectivity of various Chlorella species and the nature of their cell wall components, algae-free P. bursaria cells were mixed with 15 strains of cultivated Chlorella species and observed for the establishment of endosymbiosis at 1 h and 3 weeks after mixing. Only 2 free-living algal strains, C. sorokiniana C-212 and C. kessleri C-531, were maintained in the host cells, whereas free-living C. sorokiniana C-43, C. kessleri C-208, C. vulgaris C-27, C. ellipsoidea C-87 and C-542, C. saccharophila C-183 and C-169, C. fusca var. vacuolata C-104 and C-28, C. zofingiensis C-111, and C. protothecoides C-150 and C-206 and the cultivated symbiotic Chlorella sp. strain C-201 derived from Spongilla fluviatilis could not be maintained. These infection-incapable strains could escape from the host digestive vacuole but failed to localize beneath the host cell membrane and were eventually digested. Labeling of their cell walls with Alexa Fluor 488-conjugated wheat germ agglutinin, GS-II, or concanavalin A, with or without pretreatment with 0.4 N NaOH, showed no relationship between their infectivity and the stainability with these lectins. Our results indicate that the infectivity of Chlorella species for P. bursaria is not based on the sugar residues on their cell wall and on the alkali-insoluble part of the cell wall components, but on their ability to localize just beneath the host cell membrane after escaping from the host digestive vacuole. |
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Authors:
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Yuuki Kodama; Masahiro Fujishima |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-07-03 |
Journal Detail:
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Title: Protoplasma Volume: 231 ISSN: 0033-183X ISO Abbreviation: Protoplasma Publication Date: 2007 |
Date Detail:
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Created Date: 2007-07-02 Completed Date: 2007-11-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806853 Medline TA: Protoplasma Country: Austria |
Other Details:
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Languages: eng Pagination: 55-63 Citation Subset: IM |
Affiliation:
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Department of Natural Science and Symbiosis, Graduate School of Science and Engineering, Yamaguchi University, Yamaguchi, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrate Metabolism* / drug effects Cell Membrane / drug effects, metabolism* Cell Wall / chemistry*, drug effects Chlorella / cytology, drug effects, isolation & purification, pathogenicity* Concanavalin A / pharmacology Lectins / metabolism Paramecium / cytology, drug effects, microbiology* Staining and Labeling Symbiosis / drug effects Vacuoles / drug effects, microbiology* Wheat Germ Agglutinins / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Lectins; 0/Wheat Germ Agglutinins; 11028-71-0/Concanavalin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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