Document Detail


Infection with prevalent clinical strains of Mycobacterium tuberculosis leads to differential maturation of monocyte derived dendritic cells.
MedLine Citation:
PMID:  18336920     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The link between innate and adaptive host immune response to Mycobacterium tuberculosis (M.tb) is driven by dendritic cells (DC). In this study, we examined the ability of prevalent clinical strains from south India (S7, S10) and laboratory strain H37Rv (Rv) to induce maturation of monocyte derived dendritic cells (MoDC). The phenotypic and functional changes of DC upon infection with different strains of M.tb were evaluated. It was observed that S7 and Rv strains partially hampered the maturation of MoDC as reflected by the low expression of maturation markers and co-stimulatory markers when compared to LPS stimulated MoDC. In contrast, strain S10 infected DC showed a marked increase in the expression of these markers. The functional property was investigated by the ability of infected MoDC to induce T-cell proliferation and to stimulate secretion of IFN-gamma by CD4(+)T-cells. It was found that Rv and S7 infected MoDC were less efficient in inducing T-cell proliferation. The secretion of IL-12 by Rv and S7 infected MoDC was also found to be significantly lesser than LPS stimulated MoDC. On the other hand, S10 infected MoDC showed enhanced T-cell stimulation and cytokine secretion. Together these results indicate that there is a substantial variability in the capacity of M.tb clinical strains to induce maturation of DC which may be dependent upon their virulence.
Authors:
P Rajashree; Sulochana D Das
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-02-21
Journal Detail:
Title:  Immunology letters     Volume:  117     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-08     Completed Date:  2008-07-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  174-80     Citation Subset:  IM    
Affiliation:
Department of Immunology, Tuberculosis Research Centre (ICMR), Mayor VR Ramanathan Road, Chetpet, Chennai 600031, India.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Cell Differentiation / immunology*
Cells, Cultured
Cytokines / secretion
Dendritic Cells / cytology,  immunology*
Female
Humans
Immunity, Cellular*
Immunophenotyping
Lymphocyte Activation
Male
Middle Aged
Monocytes / cytology,  immunology
Mycobacterium tuberculosis / immunology*,  pathogenicity
Species Specificity
T-Lymphocytes / immunology
Tuberculosis / immunology*,  microbiology
Chemical
Reg. No./Substance:
0/Cytokines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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